Astaxanthin protects against MPTP/MPP+-induced mitochondrial dysfunction and ROS production in vivo and in vitro.
Astaxanthin protects against MPTP/MPP+-induced mitochondrial dysfunction and ROS production in vivo and in vitro.
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DOI：
10.1016/j.fct.2010.10.029
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发表时间：
2011-01
影响因子：
4.3
通讯作者：
Lee, Yong J.
中科院分区：
文献类型：
--
作者：
Lee, Dae-Hee;Kim, Cuk-Seong;Lee, Yong J.
Astaxanthin (AST) is a powerful antioxidant that occurs naturally in a wide variety of living organisms. We have investigated the role of AST in preventing 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced apoptosis of the substantia nigra (SN) neurons in the mouse model of Parkinson’s disease (PD) and 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity of SH-SY5Y human neuroblastoma cells. In in vitro study, AST inhibits MPP+-induced production of intracellular reactive oxygen species (ROS) and cytotoxicity in SH-SY5Y human neuroblastoma cells. Preincubation of AST (50 μM) significantly attenuates MPP+-induced oxidative damage. Furthermore, AST is able to enhance the expression of Bcl-2 protein but reduce the expression of α-synuclein and Bax, and suppress the cleavage of caspase-3. Our results suggest that the protective effects of AST on MPP+-induced apoptosis may be due to its anti-oxidative properties and anti-apoptotic activity via induction of expression of superoxide dismutase (SOD) and catalase and regulating the expression of Bcl-2 and Bax. Pretreatment with AST (30mg /kg) markedly increases tyrosine hydroxylase (TH)-positive neurons and decreases the argyrophilic neurons compared with the MPTP model group. In summary, AST shows protection from MPP+/MPTP-induced apoptosis in the SH-SY5Y cells and PD model mouse SN neurons, and this effect may be attributable to upregulation of the expression of Bcl-2 protein, downregulation of the expression of Bax and α-synuclein, and inhibition of the activation of caspase-3. These data indicate that AST may provide a valuable therapeutic strategy for the treatment of progressive neurodegenerative disease such as Parkinson’s disease.
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DOI：
10.1016/0006-291x(86)91210-6
发表时间：
1986-05-29
影响因子：
3.1
作者：
DIMONTE, D;SANDY, MS;SMITH, MT
通讯作者：
SMITH, MT
影响因子：
2.9
作者：
JYONOUCHI, H;ZHANG, L;TOMITA, Y
通讯作者：
TOMITA, Y
影响因子：
3.2
作者：
Bi, Jing;Wang, Xiao-bo;Guo, Lei
通讯作者：
Guo, Lei
影响因子：
4.8
作者：
BENDICH, A;OLSON, JA
通讯作者：
OLSON, JA
影响因子：
4.8
作者：
da Costa, CA;Paitel, E;Checler, F
通讯作者：
Checler, F