Preparation and Coculture of Neurons and Glial Cells
Preparation and Coculture of Neurons and Glial Cells
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神经元和胶质细胞的制备和共培养
DOI：
10.1002/0471143030.cb0207s32
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发表时间：
2006
影响因子：
--
通讯作者：
B. Viviani
中科院分区：
文献类型：
--
作者：
B. Viviani
神经元、胶质细胞、星形胶质细胞和星形胶质细胞之间的密切关系在调节神经毒性作用和传播脑中随之发生的退行性事件中起相关作用。在暴露于神经毒物后，神经胶质细胞通常被激活以释放负责损伤传播的物质。这种介质的产生是神经死亡和小胶质细胞与星形胶质细胞之间通讯的结果。因为它们简单且高度可控，神经系统不同细胞的共培养物是研究这些复杂关系的有价值的工具。本单元描述了建立神经胶质细胞和小胶质细胞-星形胶质细胞三明治共培养的协议，其中选定的细胞群在两个不同的表面上生长。该系统的优点在于，两个细胞群可以在暴露的任何点分离，而不会破坏细胞的完整性和组织
The close relationship between neurons, glia, astroglia, and astrocytes plays a relevant role in modulating a neurotoxic effect and propagating the consequent degenerative event in the brain. After exposure to a neurotoxicant, glial cells are often activated to release substances responsible for the propagation of the damage. the production of such mediators is the result of both neural death and communication between microglia and astrocytes. Because they are simple and highly controlled, cocultures of different cells of the nervous system are a valuable tool in the investigations of these complex relationships. This unit describes protocols to set up neuron‐glia and microglia‐astrocyte sandwich cocultures, in which the selected cell populations grow on two different surfaces. This system has the advantage that the two cell populations can be separated at any point in the exposure without disrupting the cells integrity and organization
DOI：
10.1073/pnas.88.15.6819
发表时间：
1991-08-01
影响因子：
11.1
作者：
STEWARD, O;TORRE, ER;LOTHMAN, E
通讯作者：
LOTHMAN, E
影响因子：
2.2
作者：
DENIZOT, F;LANG, R
通讯作者：
LANG, R