Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells.
Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells.
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DOI：
10.3892/etm.2021.10328
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发表时间：
2021-08
影响因子：
2.7
通讯作者：
Niu X
中科院分区：
文献类型：
--
作者：
Yan Y;Liu X;Li X;Zhou X;Yang C;Tian J;Pu J;Niu X
本研究的目的是探讨醛固酮对人主动脉平滑肌细胞（HA-VSMC）凋亡的影响，并确定fibulin-5在醛固酮诱导的HA-VSMC细胞凋亡中的作用。通过构建fibulin-5真核过表达载体和短发夹RNA干扰质粒，分别实现fibulin-5的过表达和沉默。随后确定了 fibulin-5 在醛固酮诱导的 HA-VSMC 细胞凋亡中的作用。 fibulin-5 的过度表达抑制细胞凋亡，特别是在低浓度醛固酮时；高浓度醛固酮对细胞凋亡的影响较小。 fibulin-5敲低促进高浓度醛固酮诱导的细胞凋亡，但对低浓度醛固酮诱导的细胞凋亡影响较小。因此，目前的研究结果表明fibulin-5抑制醛固酮诱导的HA-VSMC细胞凋亡，并且这种作用可能通过改变醛固酮浓度来改变。
The aim of the current study was to investigate the effect of aldosterone on apoptosis in human aortic smooth muscle cells (HA-VSMC) and to determine the role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC cells. Through the construction of a fibulin-5 eukaryotic overexpression vector and a short hairpin RNA interference plasmid, fibulin-5 was overexpressed and silenced, respectively. The role of fibulin-5 in the aldosterone-induced apoptosis of HA-VSMC was subsequently determined. The overexpression of fibulin-5 inhibited the apoptosis of cells, particularly at low concentrations of aldosterone; a smaller effect on apoptosis was induced by high concentrations of aldosterone. fibulin-5 knockdown promoted the apoptosis of cells induced by high concentrations of aldosterone but had a smaller effect on the apoptosis of cells induced by low concentrations of aldosterone. Therefore, the results of the current study indicate that fibulin-5 inhibits the aldosterone-induced apoptosis of HA-VSMC cells and that this effect may be altered by changing the aldosterone concentration.
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