Evaluation of Intestinal Microbial Metabolites in Preterm Infants with Different Initial Feeding Methods by In Vitro Fermentation Modeling System.
Evaluation of Intestinal Microbial Metabolites in Preterm Infants with Different Initial Feeding Methods by In Vitro Fermentation Modeling System.
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体外发酵模型系统评价不同初始喂养方式的早产儿肠道微生物代谢产物
DOI：
10.3390/microorganisms10071453
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发表时间：
2022-07-19
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
我们旨在通过无创方法探索早产儿肠道微生物代谢物，并分析初始喂养方式的影响。妊娠周数低于34周的早产儿每7天收集一次粪便样本。检测粪便pH、氨、胆汁酸和分泌IgA （sIgA）。将一种1:10的粪浆接种到含有不同碳水化合物的培养基中，作为唯一的碳源：乳糖（LAT）、低聚果糖（FOS）、低聚半乳糖（GOS）和2′-聚焦乳糖（FL2）。通过体外发酵系统厌氧培养24 h后，测定发酵罐内气压差、碳水化合物降解率和短链脂肪酸（SCFAs）含量。将早产儿分为两组：A组为母乳喂养早产儿，包括母乳和供体母乳（DHM）；B组：前3天采用早产儿配方奶粉喂养，第4天至出院采用母乳喂养（包括母乳和DHM）。A组90例，B组70例。A组粪便pH （p = 0.023）、氨（p = 0.001）和胆汁酸（p = 0.025）均较低。B组粪便sIgA水平也高于OD （p = 0.046）和浓度（p < 0.0001）。A组的碳水化合物降解率高于B组，尤其是LAT培养基（p = 0.017）和GOS培养基（p = 0.005）。四种介质的产气量无显著差异。A组4种不同培养基中几种不同scfa含量均高于B组，但戊酸含量低于A组。初始喂养方式对早产儿肠道微生态及微生物代谢物的影响可能至少持续数周。
We aim to explore the intestinal microbial metabolites in preterm infants with noninvasive methods and analyze the effects of initial feeding methods. Preterm infants with gestational weeks lower than 34 were recruited for fecal sample collection every 7 days. Fecal pH, ammonia, bile acid, and secretory IgA (sIgA) were tested. A 1:10 fecal slurry was inoculated into different culture media containing different carbohydrates as the only carbon source: lactose (LAT), fructooligosaccharide (FOS), galactooligosaccharide (GOS), and 2′-fucosyllactose (FL2). After 24 h of anaerobic culture through an in vitro fermentation system, air pressure difference, carbohydrate degradation rate, and short-chain fatty acids (SCFAs) content in fermentation pots were measured. Preterm infants were assigned into two groups: group A, preterm infants fed by human milk, including mother’s own milk and donor human milk (DHM); group B, preterm infants fed by preterm formula at first 3 days and fed by human milk (including mother’s own milk and DHM) from day 4 to discharge. Group A included 90 samples and group B included 70 samples. Group A had lower fecal pH (p = 0.023), ammonia (p = 0.001), and bile acids (p = 0.025). Group B also had higher fecal sIgA levels, both in OD (p = 0.046) and concentration (p < 0.0001) methods. Carbohydrates degradation rates in group A were higher than group B, especially in LAT medium (p = 0.017) and GOS medium (p = 0.005). Gas production amount had no significant difference in all four media. Several different SCFAs in four kinds of different culture media in group A were higher than in group B, but valeric acid was lower in group A. The initial feeding methods may affect the preterm infants’ intestinal microecology and microbial metabolites for at least several weeks.
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影响因子：
30.3
作者：
Kelly CJ;Zheng L;Campbell EL;Saeedi B;Scholz CC;Bayless AJ;Wilson KE;Glover LE;Kominsky DJ;Magnuson A;Weir TL;Ehrentraut SF;Pickel C;Kuhn KA;Lanis JM;Nguyen V;Taylor CT;Colgan SP
通讯作者：
Colgan SP
DOI：
10.1111/j.1749-6632.1968.tb19033.x
发表时间：
1968-01-01
影响因子：
5.2
作者：
LEVITT, MD;INGELFINGER, FJ
通讯作者：
INGELFINGER, FJ
影响因子：
4.4
作者：
Boullier, Severine;Tanguy, Myriam;Phalipon, Armelle
通讯作者：
Phalipon, Armelle
影响因子：
3.8
作者：
Picaud, Jean-Charles
通讯作者：
Picaud, Jean-Charles
根据功能分析确定，鸡肠道微生物群在体外产生的挥发性脂肪酸水平高于人类肠道微生物群
影响因子：
4.4
作者：
Lei, Fang;Yin, Yeshi;Wang, Xin
通讯作者：
Wang, Xin