A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons.
A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons.
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蚊子小RNA基因组学资源揭示了病毒和转座子的动态进化和宿主反应。
DOI：
10.1101/gr.265157.120
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发表时间：
2021-03
期刊：
影响因子：
7
通讯作者：
Lau NC
中科院分区：
文献类型：
--
作者：
Ma Q;Srivastav SP;Gamez S;Dayama G;Feitosa-Suntheimer F;Patterson EI;Johnson RM;Matson EM;Gold AS;Brackney DE;Connor JH;Colpitts TM;Hughes GL;Rasgon JL;Nolan T;Akbari OS;Lau NC
虽然蚊子是致病性虫媒病毒的主要传播媒介，但病毒感染对蚊子健康的影响很小。这种免疫力部分是由蚊子RNA干扰（RNAi）途径引起的，该途径产生抗病毒小干扰RNA（siRNA）和Piwi相互作用RNA（piRNA）。RNAi还通过有效抑制蚊子生殖系和索马体中的转座子活性来维持基因组完整性。然而，病毒和转座子小RNA调节途径还没有在蚊子中一起系统地检查。因此，我们开发了一个整合的蚊子小RNA基因组学（MSRG）资源，分析了转座子和病毒的小RNA分布在蚊子细胞培养物和体细胞和性腺组织在四个医学上重要的蚊子物种。我们的资源捕获体细胞和性腺的小RNA表达谱在蚊子细胞培养，我们报告了一种新的蚊子保守的皮尔纳簇基因座（MCpiRCL）卫星DNA重复序列组成的进化动力学。在较大的库蚊基因组中，我们检测到皮尔纳生物合成模式的高度规则的周期性，与Piwi途径基因的扩增相一致。最后，我们的资源使得能够检测蚊子细胞中的皮尔纳和siRNA群体在对病毒感染的响应期间之间的串扰。MSRG资源将有助于解剖和打击蚊子容忍和传播虫媒病毒的能力。
Although mosquitoes are major transmission vectors for pathogenic arboviruses, viral infection has little impact on mosquito health. This immunity is caused in part by mosquito RNA interference (RNAi) pathways that generate antiviral small interfering RNAs (siRNAs) and Piwi-interacting RNAs (piRNAs). RNAi also maintains genome integrity by potently repressing mosquito transposon activity in the germline and soma. However, viral and transposon small RNA regulatory pathways have not been systematically examined together in mosquitoes. Therefore, we developed an integrated mosquito small RNA genomics (MSRG) resource that analyzes the transposon and virus small RNA profiles in mosquito cell cultures and somatic and gonadal tissues across four medically important mosquito species. Our resource captures both somatic and gonadal small RNA expression profiles within mosquito cell cultures, and we report the evolutionary dynamics of a novel Mosquito-Conserved piRNA Cluster Locus (MCpiRCL) made up of satellite DNA repeats. In the larger culicine mosquito genomes we detected highly regular periodicity in piRNA biogenesis patterns coinciding with the expansion of Piwi pathway genes. Finally, our resource enables detection of cross talk between piRNA and siRNA populations in mosquito cells during a response to virus infection. The MSRG resource will aid efforts to dissect and combat the capacity of mosquitoes to tolerate and spread arboviruses.
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影响因子：
3.8
作者：
Brustolin M;Pujhari S;Henderson CA;Rasgon JL
通讯作者：
Rasgon JL
影响因子：
2.7
作者：
Didion JP;Martin M;Collins FS
通讯作者：
Collins FS
DOI：
10.1126/science.1193162
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bartholomay LC;Waterhouse RM;Mayhew GF;Campbell CL;Michel K;Zou Z;Ramirez JL;Das S;Alvarez K;Arensburger P;Bryant B;Chapman SB;Dong Y;Erickson SM;Karunaratne SH;Kokoza V;Kodira CD;Pignatelli P;Shin SW;Vanlandingham DL;Atkinson PW;Birren B;Christophides GK;Clem RJ;Hemingway J;Higgs S;Megy K;Ranson H;Zdobnov EM;Raikhel AS;Christensen BM;Dimopoulos G;Muskavitch MA
通讯作者：
Muskavitch MA
影响因子：
4.5
作者：
Chirn GW;Rahman R;Sytnikova YA;Matts JA;Zeng M;Gerlach D;Yu M;Berger B;Naramura M;Kile BT;Lau NC
通讯作者：
Lau NC
影响因子：
3.2
作者：
Araujo, Ricardo Vieira Y.;Feitosa-Suntheimer, Fabiana;Colpitts, Tonya M.
通讯作者：
Colpitts, Tonya M.