Morphogenesis of Rete Ridges in Human Oral Mucosa: A Pioneering Morphological and Immunohistochemical Study
Morphogenesis of Rete Ridges in Human Oral Mucosa: A Pioneering Morphological and Immunohistochemical Study
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人类口腔粘膜 Rete 脊的形态发生：一项开创性的形态学和免疫组织化学研究
DOI：
10.1159/000342926
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发表时间：
2012-11
影响因子：
2.7
通讯作者：
He, Sangang
中科院分区：
文献类型：
--
作者：
Wu, Tianfu;Xiong, Xuepeng;Zhang, Wenfeng;Zou, Haixiao;Xie, Haiyan;He, Sangang
目的：组织工程化口腔粘膜（TEOM）的主要障碍之一是缺乏可削弱表皮和真皮之间连接的网脊（RR）结构。本研究旨在研究RRs的自然形态以及细胞外信号调节激酶1/2（ERK 1/2），Ki 67和角蛋白-19的表达，这些蛋白分别与口腔表皮中的细胞机械转导，增殖和干性有关。研究方法：对52例标本的咀嚼粘膜（Mm）和衬层粘膜（Lm）的RR特征，包括类型、密度、长度和宽度进行了分析。免疫组化检测ERK 1/2、Ki 67和角蛋白19的表达。ERK 1/2激活咀嚼刺激证实了在体外通过加载压力培养的角质形成细胞分离的标本。结果：RR可分为三种类型，Mm和Lm的RR不同。ERK 1/2阳性（%ERK 1/2+）基底层细胞的长度和百分比呈负相关（p = 0.004），而长度和% Ki 67+基底层细胞呈正相关（p = 0.013）。ERK 1/2+基底层细胞%和角蛋白19+基底层细胞%呈负相关（p = 0.011）。ERK 1/2在口腔上皮中的激活由压力诱导并在培养的角质形成细胞中繁殖。结论：Mm的RR较长，这可能是由于咀嚼压力通过ERK 1/2激活诱导局部基底细胞增殖和迁移。我们的研究结果初步解释了口腔咀嚼压力对RR组织形态的影响。本研究结果有助于进一步研究TEOM模型中RR的发育和重建，以增强表皮-真皮连接。
Objective: One of the major impediments in tissue-engineered oral mucosa (TEOM) is the lack of rete ridge (RR) structures that can weaken the connection between the epidermis and dermis. This study aimed to investigate the native morphology of RRs as well as the expression of extracellular signal-regulated kinase 1/2 (ERK1/2), Ki67, and keratin-19, which are related to cell mechanotransduction, proliferation, and stemness in the oral epidermis, respectively. Methods: RR characteristics, including type, density, length, and width, were analyzed in the masticatory mucosa (Mm) and lining mucosa (Lm) sites of 52 specimens. The expression of ERK1/2, Ki67, and keratin-19 was assessed by immunohistochemistry. ERK1/2 activation by masticatory stimuli was confirmed in vitro by loading pressure onto cultured keratinocytes isolated from the specimens. Results: Three types of RR were found. The RRs in the Mm and Lm differed. The length and percentage of ERK1/2-positive (%ERK1/2+) basal layer cells had a negative correlation (p = 0.004), whereas the length and %Ki67+ basal layer cells had a positive correlation (p = 0.013). The %ERK1/2+ basal layer cells and %keratin-19+ basal layer cells had a negative relationship (p = 0.011). ERK1/2 activation in the oral epithelium was induced by pressure and propagated in cultured keratinocytes. Conclusion: RRs are longer in the Mm, which may result from the topical basal cell proliferation and migration induced by masticatory pressure via ERK1/2 activation. Our findings preliminarily interpret RR histomorphology as influenced by oral masticatory pressure. Results may benefit future studies on RR development and reconstruction in TEOM models to enhance the epidermis-dermis connection.
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DOI：
10.1016/j.bbrc.2004.02.101
发表时间：
2004-04
影响因子：
3.1
作者：
M. Hofmann;Julijana Zaper;A. Bernd;J. Bereiter-Hahn;R. Kaufmann;S. Kippenberger
通讯作者：
M. Hofmann;Julijana Zaper;A. Bernd;J. Bereiter-Hahn;R. Kaufmann;S. Kippenberger
影响因子：
4
作者：
M. Michel;Natalie Török;M. Godbout;Marc Lussier;Pierrette Gaudreau;André Royal;Lucie Germain
通讯作者：
M. Michel;Natalie Török;M. Godbout;Marc Lussier;Pierrette Gaudreau;André Royal;Lucie Germain
影响因子：
5.4
作者：
Peng Zhang;Yuqiong Wu;Zong-Lai Jiang;Lingyong Jiang;B. Fang
通讯作者：
Peng Zhang;Yuqiong Wu;Zong-Lai Jiang;Lingyong Jiang;B. Fang
影响因子：
4.6
作者：
JANI, RM;BHARGAVA, K
通讯作者：
BHARGAVA, K
影响因子：
2.7
作者：
W. Lu;Jinhua Yu;Yong-jie Zhang;K. Ji;Yalei Zhou;Yuan Li;Z. Deng;Yan Jin
通讯作者：
W. Lu;Jinhua Yu;Yong-jie Zhang;K. Ji;Yalei Zhou;Yuan Li;Z. Deng;Yan Jin