Cloning and characterization of the LEF/TCF gene family in grass carp (Ctenopharyngodon idella) and their expression profiles in response to grass carp reovirus infection
Cloning and characterization of the LEF/TCF gene family in grass carp (Ctenopharyngodon idella) and their expression profiles in response to grass carp reovirus infection
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草鱼（Ctenopharyngodon idella）LEF/TCF基因家族的克隆和表征及其响应草鱼呼肠孤病毒感染的表达谱
DOI：
10.1016/j.fsi.2018.11.057
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Zhu D;Huang R;Chen L;Fu P;Luo L;He L;Li Y;Liao L;Zhu Z;Wang Y
来自高迁移率族（HMG）盒家族的T细胞因子/淋巴增强子结合因子（TCF/LEF）蛋白充当Wnt信号传导途径的主要下游效应物。HMGB蛋白在哺乳动物的免疫系统中发挥多方面的作用。为明确草鱼LEF/TCF基因的免疫学特性，对草鱼LEF/TCF基因TCF 7、LEF 1、TCF 7 L1 A、TCF 7 L1 B和TCF 7 L2进行了鉴定和分析。所有5个LEF/TCF蛋白都含有两个特征结构域：HMG-BOX结构域和CTNNB 1结合区。系统进化树分析表明，LEF/TCF蛋白代表不同的谱系。这些亚细胞定位的结果表明，四个LEF/TCF基因只定位在细胞核内，而TCF 7 L2定位在细胞质和细胞核中。这些LEF/TCF家族基因的mRNA表达谱在不同组织中存在差异。草鱼呼肠孤病毒（GCRV）感染后，肝脏中TCF 7、TCF 7 L1 A和TCF 7 L2的mRNA表达水平发生显著变化，TCF 7和TCF 7 L1 A反应较早，TCF 7 L2反应较晚。这表明这些基因可能参与GCRV相关的免疫反应。此外，TCF 7促进Bcl 6转录响应GCRV的挑战。这些发现进一步加深了我们对硬骨鱼类LEF/TCF基因功能的理解。
T-cell factor/lymphoid enhancer-binding factor (TCF/LEF) proteins from the High Mobility Group (HMG) box family act as the main downstream effectors of the Wnt signaling pathway. HMGB proteins play multifaceted roles in the immune system of mammals. To clarify the immunological characteristics of LEF/TCF genes in grass carp (Ctenopharyngodon idella), five LEF/TCF genes (TCF7, LEF1, TCF7L1A, TCF7L1B, and TCF7L2) were identified and characterized. All five LEF/TCF proteins contained two characteristic domains: a HMG-BOX domain and a CTNNB1_binding region. Phylogenetic tree analysis revealed that the LEF/TCF proteins were represented different lineages. These results of subcellular localization showed that four of the LEF/TCF genes were localized exclusively within the nucleus, while TCF7L2 was localized in the cytoplasm and nucleus. The mRNA expression profiles of these LEF/TCF family genes differed across different tissues. The mRNA expression levels of TCF7, TCF7L1A, and TCF7L2 changed significantly in liver after grass carp reovirus (GCRV) challenge; TCF7 and TCF7L1A responded early while TCF7L2 responded late. This suggests that these genes may participate in GCRV-related immune responses. Moreover, TCF7 promoted Bcl6 transcription in response to the GCRV challenge. These findings further our understanding of the function of LEF/TCF genes in teleosts.
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影响因子：
--
作者：
M. Rahmani;J. Carthy;B. McManus
通讯作者：
M. Rahmani;J. Carthy;B. McManus
DOI：
10.1016/j.biopha.2017.06.061
发表时间：
2017-09
期刊：
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
影响因子：
--
作者：
Qian Xiao;Zhengxi Chen;Xiaozhuang Jin;Runyi Mao;Zhen-qing Chen
通讯作者：
Qian Xiao;Zhengxi Chen;Xiaozhuang Jin;Runyi Mao;Zhen-qing Chen
DOI：
10.1126/science.1176676
发表时间：
2009-08-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Nurieva RI;Chung Y;Martinez GJ;Yang XO;Tanaka S;Matskevitch TD;Wang YH;Dong C
通讯作者：
Dong C
影响因子：
3.7
作者：
Alkelai A;Greenbaum L;Lupoli S;Kohn Y;Sarner-Kanyas K;Ben-Asher E;Lancet D;Macciardi F;Lerer B
通讯作者：
Lerer B
DOI：
10.4049/jimmunol.1003497
发表时间：
2011-04-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Yu Q;Sharma A;Ghosh A;Sen JM
通讯作者：
Sen JM