Siderophore activity of myo-inositol hexakisphosphate in Pseudomonas aeruginosa
Siderophore activity of myo-inositol hexakisphosphate in Pseudomonas aeruginosa
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铜绿假单胞菌中肌醇六磷酸的铁载体活性
DOI：
10.1128/jb.176.12.3455-3459.1994
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发表时间：
1994
影响因子：
3.2
通讯作者：
P. Lambert
中科院分区：
文献类型：
--
作者：
A. W. Smith;D. Poyner;H. Hughes;P. Lambert
肌醇六磷酸（InsP 6）存在于土壤和大多数（如果不是全部的话）植物和动物细胞中，据估计其对Fe 3+的亲和力在10（25）至10（30）M-1的范围内。在这份报告中，我们证明了铁InsP 6复合物具有铁载体活性，并能够逆转乙二胺二（邻羟基苯基）乙酸对铜绿假单胞菌的铁限制性生长抑制。在运输研究中使用55 Fe-InsP 6，铁的吸收受到铁的强烈调节，在充满铁的条件下生长后被抑制，并通过氰化钾和羰基氰间氯苯腙处理抑制。铁转运的动力学显示Km为100 nM。通过InsP 6将[3 H] InsP 6与分离的膜结合的自置换揭示了单一类别的结合位点（Kd = 143 +/- 6 nM;希尔系数，1.1 +/- 0.1）。[3 H] InsP 6与细胞膜的结合不依赖于细胞是否在高或低铁浓度的条件下生长。我们认为这是首次报道肌醇多磷酸在原核细胞中的活性。
myo-Inositol hexakisphosphate (InsP6), which is found in soil and most, if not all, plant and animal cells, has been estimated to have an affinity for Fe3+ in the range of 10(25) to 10(30) M-1. In this report, we demonstrate that the Fe-InsP6 complex has siderophore activity and is able to reverse the iron-restricted growth inhibition of Pseudomonas aeruginosa by ethylene diamine di(o-hydroxyphenyl)acetic acid. With 55Fe-InsP6 in transport studies, iron uptake is strongly iron regulated, being repressed after growth in iron-replete conditions and inhibited by treatment with potassium cyanide and carbonyl cyanide m-chlorophenylhydrazone. The kinetics of iron transport revealed a Km of 100 nM. Self-displacement of binding of [3H]InsP6 to isolated membranes by InsP6 revealed a single class of binding sites (Kd = 143 +/- 6 nM; Hill coefficient, 1.1 +/- 0.1). The binding of [3H]InsP6 to membranes was not dependent on whether cells had been grown under conditions of high or low iron concentrations. We believe that this is the first report of inositol polyphosphate activity in prokaryotic cells.
肌醇多磷酸受体和网格蛋白组装蛋白 AP-2 是在平面脂质双层中形成钾选择性离子通道的相关蛋白。
DOI：
10.1073/pnas.89.19.8976
发表时间：
1992
影响因子：
11.1
作者：
Timerman,AP;Mayrleitner,MM;Lukas,TJ;Chadwick,CC;Saito,A;Watterson,DM;Schindler,H;Fleischer,S
通讯作者：
Fleischer,S
肌醇六磷酸受体被鉴定为网格蛋白组装蛋白 AP-2。
DOI：
10.1016/s0006-291x(05)81473-1
发表时间：
1992
影响因子：
3.1
作者：
Voglmaier,SM;Keen,JH;Murphy,JE;Ferris,CD;Prestwich,GD;Snyder,SH;Theibert,AB
通讯作者：
Theibert,AB