Oligomerization and transport of the envelope protein of Moloney murine leukemia virus-TB and of ts1, a neurovirulent temperature-sensitive mutant of MoMuLV-TB.
Oligomerization and transport of the envelope protein of Moloney murine leukemia virus-TB and of ts1, a neurovirulent temperature-sensitive mutant of MoMuLV-TB.
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莫洛尼鼠白血病病毒-TB 和 ts1（MoMuLV-TB 的神经毒性温度敏感突变体）包膜蛋白的寡聚化和转运。
DOI：
10.1016/0042-6822(91)90438-h
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发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
Wong,PK
中科院分区：
文献类型：
--
作者：
Kamps,CA;Lin,YC;Wong,PK
Ts 1是莫洛尼鼠白血病病毒-TB（MoMuLV-TB）的温度敏感突变体，在易感品系的小鼠中引起进行性后肢麻痹疾病。以前，它已被证明，一个单一的氨基酸取代，瓦尔-25→Ile在gPr 80 env，是负责的温度敏感性，低效的运输，和gPr 80 env在限制性温度和神经毒力ofts 1的加工。由于ts 1的神经毒力与gPr 80 env的低效转运和加工相关，并且由于在涉及病毒包膜蛋白的其他系统中，已经表明包膜单体的正确折叠和寡聚化是有效转运所必需的，因此我们研究了源自野生型MoMuLV-TB或ts 1的gPr 80 env缔合成寡聚复合物的能力。在这些实验中，我们确定在限制性和非限制性温度下，来自MoMuLV-TB的gPr 80 env分子缔合形成寡聚复合物，并且这些寡聚物最有可能是三聚体。衍生自tsl的gPr 80 env分子也在两种温度下低聚;然而，在限制性温度下，三聚体复合物内的大部分分子保持为gPr 80 env，并且不加工成gp 70和Prpl 5E。这些结果表明gPr 80 env的寡聚化的缺乏不是ts 1的转运缺陷的原因。因此，通过与靶细胞内的关键位点特异性相互作用，突变体gPr 80 env的寡聚体而不是单体病毒包膜蛋白的“缠结”可能参与由ts 1产生的神经退行性疾病。
Ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB (MoMuLV-TB), causes a progressive hindlimb paralytic disease in susceptible strains of mice. Previously, it has been shown that a single amino acid substitution, Val-25→Ile in gPr80env, is responsible for the temperature sensitivity, inefficient transport, and processing of gPr80envat the restrictive temperature and the neurovirulence ofts1. Since the neurovirulence ofts1 is associated with inefficient transport and processing of gPr80envand since in other systems involving viral envelope proteins it has been shown that correct folding and oligomerization of envelope monomers are required for efficient transport, we have investigated the ability of gPr80envderived from either wild-type MoMuLV-TB orts1 to associate into oligomeric complexes. In these experiments, we establish that at both the restrictive and the nonrestrictive temperatures gPr80envmolecules derived from MoMuLV-TB associate to form oligomeric complexes and these oligomers are most likely trimers. gPr80envmolecules derived from tsl also oligomerize at both temperatures; however, at the restrictive temperature, most of the molecules within the trimeric complexes remain as gPr80envand are not processed to gp70 and Prpl 5E. These results indicate that lack of oligomerization of gPr80envis not responsible for the transport defect ofts1. Therefore, by interacting specifically with critical sites within target cells, oligomers of mutant gPr80envrather than “tangles” of monomeric viral envelope proteins may be involved in the neurodegenerative disorder produced byts1.
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ts1 是莫洛尼鼠白血病病毒 TB 的突变体，可导致 BALB/c 小鼠免疫缺陷和神经系统疾病。
影响因子：
3.7
作者：
Wong,PK;Prasad,G;Hansen,J;Yuen,PH
通讯作者：
Yuen,PH
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影响因子：
3.7
作者：
Schawaller,M;Smith,GE;Skehel,JJ;Wiley,DC
通讯作者：
Wiley,DC
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影响因子：
3.1
作者：
B. Brooks;J. R. Swarz;O. Narayan;R. Johnson
通讯作者：
R. Johnson
用于分离莫洛尼白血病病毒自发温度敏感突变体的快速、选择性程序。
影响因子：
3.7
作者：
P. Wong;L. J. Russ;J. McCarter
通讯作者：
J. McCarter
DOI：
10.1016/s0021-9258(19)45358-1
发表时间：
1982-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Brigitte Saunier;R. Kilker;Jan S. Tkaczq;Andrea Quaronill;A. Herscovics
通讯作者：
Brigitte Saunier;R. Kilker;Jan S. Tkaczq;Andrea Quaronill;A. Herscovics