Dual detection of cancer biomarker CA125 using absorbance and electrochemical methods.
Dual detection of cancer biomarker CA125 using absorbance and electrochemical methods.
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DOI：
10.1039/c3an00668a
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发表时间：
2013-10-07
期刊：
影响因子：
--
通讯作者：
Wu N
中科院分区：
文献类型：
--
作者：
Al-Ogaidi I;Aguilar ZP;Suri S;Gou H;Wu N
基于双重信号转导机制的酶联免疫分析法已被开发用于检测卵巢癌生物标志物CA 125。免疫测定使用纳米电极阵列（NEA）芯片和吸光度法进行双重检测。NEA用于确认在高结合96孔板中进行的CA 125光学检测。使用碱性磷酸酶（AP）标记检测抗体以允许CA 125的光学和电化学检测。AP酶催化两种底物。对硝基苯基磷酸盐（PNPP）产生显色对硝基苯酚（PNP），可在405 nm处进行光学检测。对氨基苯磷酸盐（PAPP）产生电活性对氨基苯酚（PAP），可在−0.1至0.3 V之间通过安培法检测。光学和电化学免疫测定的线性范围分别为5-1000 U/mL和5-1000 U/mL。光学和电化学方法的检测限分别为1.3 U/mL和40 U/mL。
An enzyme-linked immunoassay based on the dual signal transduction mechanisms has been developed for detection of ovarian cancer biomarker CA125. The immunoassays use a nanoelectrode array (NEA) chip and absorbance methods for the dual detection. The NEA is used to confirm the optical detection of CA125 that is carried out in a high-binding 96-well plate. An alkaline phosphatase (AP) enzyme was used to label the detection antibody to allow for both the optical and electrochemical detection of CA125. Two kinds of substrates were catalyzed by the AP enzyme. Para-nitrophenylphosphate (PNPP) produces chromogenic para-nitrophenol (PNP), which can be optically detected at 405 nm. Para-aminophenylphosphate (PAPP) produces electroactive para-aminophenol (PAP), which can be detected amperometrically between −0.1 to 0.3 V. The linear ranges have been determined to be 5–1000 U/mL and 5–1000 U/mL for the optical and electrochemical immunoassays, respectively. The limit of detection of the optical immunoassay is 1.3 U/mL and 40 U/mL for the optical and electrochemical methods, respectively.
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影响因子：
3.7
作者：
Emmanuel C;Gava N;Kennedy C;Balleine RL;Sharma R;Wain G;Brand A;Hogg R;Etemadmoghadam D;George J;Australian Ovarian Cancer Study Group;Birrer MJ;Clarke CL;Chenevix-Trench G;Bowtell DD;Harnett PR;deFazio A
通讯作者：
deFazio A
影响因子：
45.3
作者：
Santillan, A;Garg, R;Bristow, RE
通讯作者：
Bristow, RE
影响因子：
2.9
作者：
Whisnant, AR;Gilman, SD
通讯作者：
Gilman, SD
影响因子：
15.9
作者：
BAST, RC;FEENEY, M;KNAPP, RC
通讯作者：
KNAPP, RC
影响因子：
4.3
作者：
Li, Huaqing;Low, Jeremy;Wu, Nianqiang
通讯作者：
Wu, Nianqiang