Concurrent Detection of Human Norovirus and Bacterial Pathogens in Water Samples from an Agricultural Region in Central California Coast.
Concurrent Detection of Human Norovirus and Bacterial Pathogens in Water Samples from an Agricultural Region in Central California Coast.
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同时检测来自加利福尼亚中部海岸农业地区的水样中的人类诺如病毒和细菌病原体。
DOI：
10.3389/fmicb.2017.01560
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发表时间：
2017
影响因子：
5.2
通讯作者：
Cooley MB
中科院分区：
文献类型：
--
作者：
Tian P;Yang D;Shan L;Wang D;Li Q;Gorski L;Lee BG;Quiñones B;Cooley MB
细菌病原体和人类诺如病毒(HuNoV)是由受污染的食物和水引起的急性胃肠炎的主要原因。公共水道可能受到各种来源的污染，并在暴雨事件后发生洪水，导致农产品田地受到病原体污染。我们在加利福尼亚州中部海岸的一个主要绿色农产品生产区对几个公共分水岭进行了调查，以确定胡诺夫新城疫病毒和细菌病原体的流行情况。使用摩尔棉签每两个月在该地区30多个采样点收集环境样本。该地区环境水样中检出高流行的新城疫病毒和细菌病原体。其中，新城疫、O157志贺氏菌、非O157志贺氏菌、沙门氏菌和李斯特菌的总检出率分别为25.58%、7.91%、9.42%、59.65%和44.30%。春季沙门氏菌和单核细胞增多性李斯特氏菌的检出率明显较高。秋季和春季的O157 STEC的检出率较高。秋季非O157 STEC的总体检出率低于其他季节，但差异不显着。湖北新城疫总体检出率秋季最高，春季和冬季次之，夏季最低，明显低于其他季节。这项研究首次评估了环境水中胡诺夫新城疫病毒的检出率与四种细菌病原体的检出率之间的相关性。总体而言，在检测四种细菌病原体的阳性或阴性样本中，HuNov的检测率没有显著差异。对动物和人类影响地区的病原体进行了调查。在受动物影响的地区，细菌病原体的检测率明显高于受人类影响的地区。然而，这两个地区的人间新城疫病毒发现率没有差异。普通大肠埃希菌的总体检出水平与虎诺氏新城疫病毒的检出无相关性。
Bacterial pathogens and human norovirus (HuNoV) are major cause for acute gastroenteritis caused by contaminated food and water. Public waterways can become contaminated from a variety of sources and flood after heavy rain events, leading to pathogen contamination of produce fields. We initiated a survey of several public watersheds in a major leafy green produce production region of the Central California Coast to determine the prevalence of HuNoV as well as bacterial pathogens. Moore swabs were used to collect environmental samples bi-monthly at over 30 sampling sites in the region. High prevalence of HuNoV and bacterial pathogens were detected in environmental water samples in the region. The overall detection rates of HuNoV, O157 Shiga toxin-producing Escherichia coli (STEC), non-O157 STEC, Salmonella, and Listeria were 25.58, 7.91, 9.42, 59.65, and 44.30%, respectively. The detection rates of Salmonella and L. monocytogenes were significantly higher in the spring. Fall and spring had elevated detection rates of O157 STEC. The overall detection rates of non-O157 STEC in the fall were lower than the other seasons but not significant. The overall detection rates of HuNoV were highest in fall, followed by spring and winter, with summer being lowest and significantly lower than other seasons. This study presented the first study of evaluating the correlation between the detection rate of HuNoV and the detection rates of four bacterial pathogens from environmental water. Overall, there was no significant difference in HuNoV detection rates between samples testing positive or negative for the four bacterial pathogens tested. Pathogens in animal-impacted and human-impacted areas were investigated. There were significant higher detection rates in animal-impacted areas than that of human-impacted areas for bacterial pathogens. However, there was no difference in HuNoV detection rates between these two areas. The overall detection levels of generic E. coli and detection rate of HuNoV showed no correlation.
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DOI：
10.1016/j.scitotenv.2014.05.072
发表时间：
2014-08-15
期刊：
The Science of the total environment
影响因子：
--
作者：
Corsi SR;Borchardt MA;Spencer SK;Hughes PE;Baldwin AK
通讯作者：
Baldwin AK
DOI：
10.1016/j.ijfoodmicro.2013.04.003
发表时间：
2013-07-01
影响因子：
5.4
作者：
Benjamin, Lisa;Atwill, Edward R.;Mandrell, Robert E.
通讯作者：
Mandrell, Robert E.
DOI：
10.1016/j.ijfoodmicro.2015.02.029
发表时间：
2015-06-02
影响因子：
5.4
作者：
Hennechart-Collette, Catherine;Martin-Latil, Sandra;Perelle, Sylvie
通讯作者：
Perelle, Sylvie
影响因子：
6.4
作者：
KAPLAN, JE;GOODMAN, RA;GARY, GW
通讯作者：
GARY, GW
影响因子：
120.7
作者：
Beller, M;Ellis, A;Spika, JS
通讯作者：
Spika, JS