Innate immune response to Mycobacterium tuberculosis Beijing and other genotypes.
Innate immune response to Mycobacterium tuberculosis Beijing and other genotypes.
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DOI：
10.1371/journal.pone.0013594
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发表时间：
2010-10-25
期刊：
影响因子：
3.7
通讯作者：
Neyrolles O
中科院分区：
文献类型：
--
作者：
Wang C;Peyron P;Mestre O;Kaplan G;van Soolingen D;Gao Q;Gicquel B;Neyrolles O
作为一个物种，结核分枝杆菌比以前想象的更加多样化。特别是，北京结核分枝杆菌菌株家族正在世界范围内传播和进化，这引起了公共卫生问题。最近进一步描述了该家族内的遗传多样性，并且一种称为 Bmyc10 的特定基因型已被证明代表了世界多个地区所有北京临床分离株的 60% 以上。宿主免疫系统如何感知和响应各种结核分枝杆菌菌株可能会深刻影响不同分枝杆菌谱系的临床结果和相对流行病学成功。我们假设 Bmyc10 组的成功可能至少部分依赖于其改变先天免疫反应以及宿主吞噬细胞分泌细胞因子和趋化因子的能力。我们用一系列基因明确的结核分枝杆菌临床分离株感染人类巨噬细胞和树突状细胞，这些临床分离株属于不同的分枝杆菌科，包括北京分枝杆菌科。我们使用抗体阵列在半全局水平上分析了细胞因子和趋化因子的分泌，从而可以检测 65 种免疫相关的可溶性分子。我们的数据表明，北京菌株诱导的白细胞介素（IL）-6、肿瘤坏死因子（TNF）、IL-10和GRO-α明显低于H37Rv参考菌株，这一特征为其他现代和古代结核分枝杆菌家族所共有，并且构成了整个北京家族的特征。然而，北京菌株在调节细胞因子分泌的能力方面并无差异。我们的结果证实并扩展了先前的报告，表明结核分枝杆菌北京菌株总体上在人类吞噬细胞中是较差的体外细胞因子诱导剂。结果表明，北京Bmyc10在流行病学上的成功不太可能依赖于这组菌株损害抗分枝杆菌先天免疫的任何特定能力。
As a species, Mycobacterium tuberculosis is more diverse than previously thought. In particular, the Beijing family of M. tuberculosis strains is spreading and evoluating throughout the world and this is giving rise to public health concerns. Genetic diversity within this family has recently been delineated further and a specific genotype, called Bmyc10, has been shown to represent over 60% of all Beijing clinical isolates in several parts of the world. How the host immune system senses and responds to various M. tuberculosis strains may profoundly influence clinical outcome and the relative epidemiological success of the different mycobacterial lineages. We hypothesised that the success of the Bmyc10 group may, at least in part, rely upon its ability to alter innate immune responses and the secretion of cytokines and chemokines by host phagocytes. We infected human macrophages and dendritic cells with a collection of genetically well-defined M. tuberculosis clinical isolates belonging to various mycobacterial families, including Beijing. We analyzed cytokine and chemokine secretion on a semi-global level using antibody arrays allowing the detection of sixty-five immunity-related soluble molecules. Our data indicate that Beijing strains induce significantly less interleukin (IL)-6, tumor necrosis factor (TNF), IL-10 and GRO-α than the H37Rv reference strain, a feature that is variously shared by other modern and ancient M. tuberculosis families and which constitutes a signature of the Beijing family as a whole. However, Beijing strains did not differ relative to each other in their ability to modulate cytokine secretion. Our results confirm and expand upon previous reports showing that M. tuberculosis Beijing strains in general are poor in vitro cytokine inducers in human phagocytes. The results suggest that the epidemiological success of the Beijing Bmyc10 is unlikely to rely upon any specific ability of this group of strains to impair anti-mycobacterial innate immunity.
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影响因子：
9.4
作者：
Kong, Y.;Cave, M. D.;Yang, Z. H.
通讯作者：
Yang, Z. H.
影响因子：
3.2
作者：
Filliol, I;Motiwala, AS;Alland, D
通讯作者：
Alland, D
影响因子：
4.6
作者：
Chacón-Salinas, R;Serafín-López, J;Estrada-García, I
通讯作者：
Estrada-García, I
影响因子：
3.7
作者：
Dos Vultos T;Mestre O;Rauzier J;Golec M;Rastogi N;Rasolofo V;Tonjum T;Sola C;Matic I;Gicquel B
通讯作者：
Gicquel B
DOI：
10.1073/pnas.091096998
发表时间：
2001-05-08
影响因子：
11.1
作者：
Manca, C;Tsenova, L;Kaplan, G
通讯作者：
Kaplan, G