Molecular recognition by a polymorphic cell surface receptor governs cooperative behaviors in bacteria.
Molecular recognition by a polymorphic cell surface receptor governs cooperative behaviors in bacteria.
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多态细胞表面受体的分子识别控制细菌中的合作行为。
DOI：
10.1371/journal.pgen.1003891
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发表时间：
2013-11
期刊：
影响因子：
4.5
通讯作者：
Wall D
中科院分区：
文献类型：
--
作者：
Pathak DT;Wei X;Dey A;Wall D
细胞-细胞识别是细胞协调多细胞行为的基本过程。一些微生物，如粘细菌，从自由生活的细胞中构建多细胞子实体。然而，细菌细胞是如何通过接触识别彼此的，目前还知之甚少。在这里，我们展示了粘细菌通过TRAA细胞表面受体之间的相互作用进行识别，这导致了外膜(OM)成分的融合和交换。OM交换在17个环境分离物中具有选择性，因为交换伙伴被解析为五个主要识别群。TraA是分子特异性的决定因素，因为：(I)交换伙伴与其PA14样多态结构域内的序列保守性相关，(Ii)TraA等位基因替换可预测地改变伙伴特异性。交换TraA等位基因还重新编程了菌株之间的社会互动，包括对运动性的调节和对菌株间杀戮的免疫。我们认为，TraA通过一种类似于线粒体融合和裂变循环的机制，帮助引导单细胞向连贯的细菌群落的转变，该机制混合内容以建立同质种群。在进化方面，TraA是一种罕见的绿胡子基因，它可以识别携带相同等位基因的其他人，从而提供有益的治疗。单个细胞如何相互识别以协作和组装功能组织是生物学中的一个基本问题。虽然多细胞特性通常与真核生物有关，但某些细菌群体也表现出复杂的多细胞行为，粘性细菌可能是最好的例证。例如，对饥饿的反应，粘细菌会聚集子实体，其中数千个细胞作为一个连贯的单位在发育和细胞分化中发挥作用。然而，粘性细菌或其他细菌如何通过细胞接触依赖的相互作用识别合作的细胞，目前还知之甚少。在这里，我们描述了一种机制，即粘细菌将同胞细胞和队列细胞与其他粘细菌分离株区分开来。我们发现分子识别是由一种名为TRAA的细胞表面受体介导的。细胞-细胞特异性涉及伙伴细胞的相互识别，并由所提出的同型TRAA相互作用所介导。识别的特异性由TRAA等位基因中发现的可变序列决定。因此，简单地在分离株之间交换TraA等位基因可以预测地改变伴侣的认知度。TraA-TraA识别反过来导致细胞之间外膜(OM)成分的融合和交换。我们认为，OM交换允许细胞进行交流，并使它们的OM蛋白质组变得同质。我们进一步认为，这些相互作用建立了一个在多细胞过程中发挥作用的紧密结合的细胞群体。
Cell-cell recognition is a fundamental process that allows cells to coordinate multicellular behaviors. Some microbes, such as myxobacteria, build multicellular fruiting bodies from free-living cells. However, how bacterial cells recognize each other by contact is poorly understood. Here we show that myxobacteria engage in recognition through interactions between TraA cell surface receptors, which leads to the fusion and exchange of outer membrane (OM) components. OM exchange is shown to be selective among 17 environmental isolates, as exchange partners parsed into five major recognition groups. TraA is the determinant of molecular specificity because: (i) exchange partners correlated with sequence conservation within its polymorphic PA14-like domain and (ii) traA allele replacements predictably changed partner specificity. Swapping traA alleles also reprogrammed social interactions among strains, including the regulation of motility and conferred immunity from inter-strain killing. We suggest that TraA helps guide the transition of single cells into a coherent bacterial community, by a proposed mechanism that is analogous to mitochondrial fusion and fission cycling that mixes contents to establish a homogenous population. In evolutionary terms, traA functions as a rare greenbeard gene that recognizes others that bear the same allele to confer beneficial treatment. How individual cells recognize each other to cooperate and assemble functional tissues is a fundamental question in biology. Although multicellularity is a trait that is typically associated with eukaryotes, certain groups of bacteria also exhibit complex multicellular behaviors, which are perhaps best exemplified by the myxobacteria. For example, in response to starvation myxobacteria will assemble fruiting bodies, wherein thousands of cells function as a coherent unit in development and cell differentiation. However, how myxobacteria, or for that matter other bacteria, recognize cooperating partnering cells through cell contact-dependent interactions is poorly understood. Here we describe a mechanism where myxobacteria distinguish sibling and cohort cells from other myxobacteria isolates. We show that molecular recognition is mediated by a cell surface receptor called TraA. Cell-cell specificity involves mutual recognition by partnering cells and is mediated by proposed homotypic TraA interactions. The specificity for recognition is determined by variable sequences found within traA alleles. Thus, simply swapping traA alleles between isolates predictably changes partner recognition. TraA-TraA recognition in turn leads to the fusion and exchange of outer membrane (OM) components between cells. We suggest that OM exchange allows the cells to communicate and become homogenous with respect to their OM proteome. We further suggest these interactions build a cohesive cell population that functions in multicellular processes.
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影响因子：
6.4
作者：
Ruhe ZC;Wallace AB;Low DA;Hayes CS
通讯作者：
Hayes CS
影响因子：
13.8
作者：
Rigden, DJ;Mello, LV;Galperin, MY
通讯作者：
Galperin, MY
DOI：
10.1073/pnas.1300627110
发表时间：
2013-04-23
影响因子：
11.1
作者：
Koskiniemi, Sanna;Lamoureux, James G.;Hayes, Christopher S.
通讯作者：
Hayes, Christopher S.
影响因子：
56.9
作者：
Hirose, Shigenori;Benabentos, Rocio;Shaulsky, Gad
通讯作者：
Shaulsky, Gad
影响因子：
2
作者：
HAMILTON, WD
通讯作者：
HAMILTON, WD