Chitin conduits modified with DNA-peptide coating promote the peripheral nerve regeneration
Chitin conduits modified with DNA-peptide coating promote the peripheral nerve regeneration
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DNA肽涂层修饰的甲壳素导管促进周围神经再生
DOI：
10.1088/1758-5090/ac3bdc
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发表时间：
2021-11
期刊：
影响因子：
9
通讯作者：
Baoguo Jiang
中科院分区：
文献类型：
--
作者：
Songyang Liu;Liping Zhou;Ci Li;Tiantian Min;Changfeng Lu;Shuai Han;Meng Zhang;Yongqiang Wen;Peixun Zhang;Baoguo Jiang
周围神经损伤是临床上常见的损伤之一，需要引起重视。以往的研究表明，使用可生物降解的甲壳素（CT）导管小间隙管化技术作为传统的神经外膜缝合术的替代品的有效性。为了提高CT导管修复PNI的有效性，我们用DNA-肽涂层对其表面进行了修饰。涂层由单链DNA（ssDNA）及其互补DNA β-肽模拟物组成。首先，我们用盐酸碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）法将ssDNA（DNA 1 + 2）固定在CT导管上，构建CT/DNA导管。EDC/NHS用于活化修饰的ssDNA的羧基，以通过酰胺键形成与CT上的伯胺直接反应。然后，在室温下，通过互补碱基配对原理将DNA 1 β-BDNF + DNA 2 β-VEGF模拟肽（RGI + KLT）结合到CT/DNA导管上，形成CT/RGI + KLT导管。当周围环境升高到一定温度（37 °C）时，CT/RGI + KLT导管实现了DNA′-肽的持续释放。体外实验表明，经DNA-肽涂层修饰的CT导管通过维持雪旺细胞的修复状态，促进其增殖和分泌。促进人脐静脉血管内皮细胞增殖和背根神经节轴突生长。在体内，CT/RGI + KLT导管促进损伤神经的再生和靶肌肉的功能恢复，这是通过血管生成和神经发生的协同作用来促进的。我们的研究将DNA和DNA-肽杂合体引入组织工程领域以修复PNI。
Peripheral nerve injury (PNI) is one of the common clinical injuries which needs to be addressed. Previous studies demonstrated the effectiveness of using biodegradable chitin (CT) conduits small gap tubulization technology as a substitute for traditional epineurial neurorrhaphy. Aiming to improve the effectiveness of CT conduits in repairing PNI, we modified their surface with a DNA-peptide coating. The coating consisted of single strand DNA (ssDNA) and its complementary DNAʹ-peptide mimics. First, we immobilize ssDNA (DNA1 + 2) on CT conduits by carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) method to construct CT/DNA conduits. EDC/NHS was used to activate carboxyl groups of modified ssDNA for direct reaction with primary amines on the CT via amide bond formation. Then, DNA1ʹ-BDNF + DNA2ʹ-VEGF mimic peptide (RGI + KLT) were bonded to CT/DNA conduits by complementary base pairing principle at room temperature to form CT/RGI + KLT conduits. When the surrounding environment rose to a certain point (37 °C), the CT/RGI + KLT conduits achieved sustainable release of DNA′-peptide. In vitro, the CT conduits modified with the DNA-peptide coating promoted the proliferation and secretion of Schwann cells by maintaining their repair state. It also promoted the proliferation of human umbilical vein vessel endothelial cells and axon outgrowth of dorsal root ganglion explants. In vivo, CT/RGI + KLT conduits promoted regeneration of injured nerves and functional recovery of target muscles, which was facilitated by the synergistic contribution of angiogenesis and neurogenesis. Our research brings DNA and DNA-peptide hybrids into the realm of tissue engineering to repair PNI.
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DOI：
10.1073/pnas.0505047102
发表时间：
2005-10-04
影响因子：
11.1
作者：
D'Andrea, LD;Iaccarino, G;Pedone, C
通讯作者：
Pedone, C
基于六氨钌 (III) 和 DNA 分子之间瞬时静电引力的通用 DNA 生物传感
影响因子：
12.6
作者：
Dai, Siya;Lu, Wei;Yao, Bo
通讯作者：
Yao, Bo
影响因子：
5.8
作者：
Rubert Pérez CM;Álvarez Z;Chen F;Aytun T;Stupp SI
通讯作者：
Stupp SI
影响因子：
30.8
作者：
Hacia, JG;Brody, LC;Collins, FS
通讯作者：
Collins, FS
影响因子：
6.2
作者：
E. Udina;F. Rodrı́guez;E. Verdú;M. Espejo;B. Gold;X. Navarro
通讯作者：
E. Udina;F. Rodrı́guez;E. Verdú;M. Espejo;B. Gold;X. Navarro