Environmental DNA (eDNA): A tool for quantifying the abundant but elusive round goby (Neogobius melanostomus).
Environmental DNA (eDNA): A tool for quantifying the abundant but elusive round goby (Neogobius melanostomus).
复制标题
环境DNA（EDNA）：一种量化丰富但难以捉摸的圆形Goby（Neogobius Melanostomus）的工具。
DOI：
10.1371/journal.pone.0191720
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Roseman EF
中科院分区：
文献类型：
--
作者：
Nevers MB;Byappanahalli MN;Morris CC;Shively D;Przybyla-Kelly K;Spoljaric AM;Dickey J;Roseman EF
环境DNA （eDNA）正在彻底改变生物多样性监测、占用估计和入侵物种的实时检测。在五大湖，一种来自黑海的入侵底栖鱼类圆虾虎鱼（Neogobius melanostomus）已经扩散到所有五个湖泊和许多支流，超越或吞噬了本地物种；然而，圆虾虎鱼丰度的估计受到行为和栖息地偏好的影响，这影响了估计其种群的可靠方法。通过将eDNA整合到对虾虎鱼监测中，可以获得更好的生物量估计。我们进行了中体实验来估计虾虎鱼DNA脱落和衰变的速率。此外，我们将eDNA与几种传统的野外采样方法进行了比较，以比较其作为替代/补充监测方法的用途。环境DNA衰变与其他鱼类相当，12°C （k = 0.043）的一级衰变低于19°C （k = 0.058）。在密歇根湖及其支流的已知入侵点（log10 4.8-6.2 CN/L）通常检测到圆虾虎鱼的eDNA，但在人工鱼类屏障上游没有检测到。密西根湖和休伦湖的传统技术（标记-再捕获、围网、诱捕）比eDNA检测到的变量更少、更多，但诱捕和eDNA是相关的（Pearson R = 0.87）。额外的现场测试将有助于将圆虾虎鱼的丰度与eDNA联系起来，从而深入了解圆虾虎鱼作为猎物的作用及其对食物网的影响。
Environmental DNA (eDNA) is revolutionizing biodiversity monitoring, occupancy estimates, and real-time detections of invasive species. In the Great Lakes, the round goby (Neogobius melanostomus), an invasive benthic fish from the Black Sea, has spread to encompass all five lakes and many tributaries, outcompeting or consuming native species; however, estimates of round goby abundance are confounded by behavior and habitat preference, which impact reliable methods for estimating their population. By integrating eDNA into round goby monitoring, improved estimates of biomass may be obtainable. We conducted mesocosm experiments to estimate rates of goby DNA shedding and decay. Further, we compared eDNA with several methods of traditional field sampling to compare its use as an alternative/complementary monitoring method. Environmental DNA decay was comparable to other fish species, and first-order decay was lower at 12°C (k = 0.043) than at 19°C (k = 0.058). Round goby eDNA was routinely detected in known invaded sites of Lake Michigan and its tributaries (range log10 4.8–6.2 CN/L), but not upstream of an artificial fish barrier. Traditional techniques (mark-recapture, seining, trapping) in Lakes Michigan and Huron resulted in fewer, more variable detections than eDNA, but trapping and eDNA were correlated (Pearson R = 0.87). Additional field testing will help correlate round goby abundance with eDNA, providing insight on its role as a prey fish and its impact on food webs.
登录
查看更多内容
影响因子：
4.4
作者：
Dell'Anno, A;Corinaldesi, C
通讯作者：
Corinaldesi, C
影响因子：
1.5
作者：
Hinlo, Rheyda;Furlan, Elise;Gleeson, Dianne
通讯作者：
Gleeson, Dianne
影响因子：
10.1
作者：
Bunnell, David B.;Barbiero, Richard P.;Weidel, Brian C.
通讯作者：
Weidel, Brian C.
影响因子：
2.2
作者：
Amberg, Jon J.;McCalla, S. Grace;Gaikowski, Mark P.
通讯作者：
Gaikowski, Mark P.
影响因子：
2.2
作者：
Chotkowski, MA;Marsden, JE
通讯作者：
Marsden, JE