Immune response to cytolethal distending toxin of Aggregatibacter actinomycetemcomitans in periodontitis patients.
Immune response to cytolethal distending toxin of Aggregatibacter actinomycetemcomitans in periodontitis patients.
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DOI：
10.1111/j.1600-0765.2009.01260.x
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发表时间：
2010-08
影响因子：
3.5
通讯作者：
Mayer MP
中科院分区：
文献类型：
--
作者：
Ando ES;De-Gennaro LA;Faveri M;Feres M;DiRienzo JM;Mayer MP
细胞致死性膨胀毒素（CDT）是由伴放线聚集杆菌产生的一种基因毒素。尽管它与发病机制有关，但对CDT的体液免疫应答知之甚少。本研究的目的是检测龈下定植和对A.放线菌共生菌引起抗CDT的反应。对牙周健康、局限性和广泛性侵袭性牙周炎和慢性牙周炎患者（n = 80）的血清进行了A.通过ELISA检测伴放线菌血清型a/B/c和每个CDT亚基（CdtA、CdtB和CdtC）。A.还分析了伴放线菌的龈下水平和CDT活性的中和。75.0%的局限型和81.8%的泛发型侵袭性牙周炎患者血清对A.伴放线菌对血清型B的反应在局限性（66.7%）和广泛性侵袭性牙周炎（54.5%）中检测到。对A的反应性伴放线菌与龈下定植相关（R = 0.75，p < 0.05）。A.伴随放线菌定殖或对血清型的应答以及免疫球蛋白G对CDT亚单位的应答。免疫球蛋白G对CdtA和CdtB的滴度在各组之间没有差异;然而，所有全身侵袭性牙周炎患者的血清对CdtC有反应。CDT的中和与CDT亚基抗体水平无关。对CdtA和CdtB的反应与受试者在A.伴放线菌感染然而，CdtC的反应被发现在血清中的广泛的，但不是本地化的侵略性牙周炎科目。全身性和局部侵袭性牙周炎受试者之间对CdtC的反应差异表明，感染菌株可以不同地表达CDT。或者，对CdtC的抗体应答可能需要多个位点的定殖。
Cytolethal distending toxin (CDT) is a genotoxin produced by Aggregatibacter actinomycetemcomitans. In spite of its association with pathogenesis, little is known about the humoral immune response against the CDT. This study aimed to test whether subgingival colonization and humoral response to A. actinomycetemcomitans would lead to a response against CDT. Sera from periodontally healthy, localized and generalized aggressive periodontitis and chronic periodontitis subjects (n = 80) were assessed for immunoglobulin G titers to A. actinomycetemcomitans serotypes a/b/c and to each CDT subunit (CdtA, CdtB and CdtC) by ELISA. A. actinomycetemcomitans subgingival levels and neutralization of CDT activity were also analyzed. Sera from 75.0% localized and 81.8% generalized aggressive periodontitis patients reacted to A. actinomycetemcomitans. A response to serotype b was detected in localized (66.7%) and generalized aggressive periodontitis (54.5%). Reactivity to A. actinomycetemcomitans correlated with subgingival colonization (R = 0.75, p < 0.05). There was no correlation between A. actinomycetemcomitans colonization or response to serotypes and the immunoglobulin G response to CDT subunits. Titers of immunoglobulin G to CdtA and CdtB did not differ among groups; however, sera of all generalized aggressive periodontitis patients reacted to CdtC. Neutralization of CDT was not correlated with levels of antibodies to CDT subunits. Response to CdtA and CdtB did not correlate with the periodontal status of the subject in the context of an A. actinomycetemcomitans infection. However, a response to CdtC was found in sera of generalized but not of localized aggressive periodontitis subjects. Differences in response to CdtC between generalized and localized aggressive periodontitis subjects indicate that CDT could be expressed differently by the infecting strains. Alternatively, the antibody response to CdtC could require the colonization of multiple sites.
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影响因子：
--
作者：
Fernandes, K. P. S.;Mayer, M. P. A.;Russo, M.
通讯作者：
Russo, M.
影响因子：
3
作者：
Purdy, D;Buswell, CM;Leach, SA
通讯作者：
Leach, SA
影响因子：
2.8
作者：
Belibasakis, Georgios N.;Brage, Monica;Johansson, Anders
通讯作者：
Johansson, Anders
影响因子：
3.1
作者：
Lewis, DA;Stevens, MK;Hansen, EJ
通讯作者：
Hansen, EJ
影响因子：
--
作者：
Kawamoto, D.;Ando, E. S.;Mayer, M. P. A.
通讯作者：
Mayer, M. P. A.