Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.
Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.
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DOI：
10.1242/dev.111054
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
St Johnston D
中科院分区：
文献类型：
--
作者：
Lowe N;Rees JS;Roote J;Ryder E;Armean IM;Johnson G;Drummond E;Spriggs H;Drummond J;Magbanua JP;Naylor H;Sanson B;Bastock R;Huelsmann S;Trovisco V;Landgraf M;Knowles-Barley S;Armstrong JD;White-Cooper H;Hansen C;Phillips RG;UK Drosophila Protein Trap Screening Consortium;Lilley KS;Russell S;St Johnston D
虽然我们现在有大量关于果蝇基因组中所有基因的转录模式的信息，但对编码蛋白质的性质知之甚少。为了提供信息的表达模式和亚细胞定位的许多蛋白质的平行，我们进行了大规模的蛋白质陷阱筛选使用的混合piggyBac载体携带人工外显子编码黄色荧光蛋白（YFP）和蛋白质亲和标签。从筛选4100万个胚胎中，我们回收了616个经验证的独立YFP阳性品系，代表374个基因中的蛋白陷阱，其中三分之二在以前的P元件蛋白陷阱筛选中没有被标记。超过20个不同的研究小组随后表征了标记蛋白在各种组织和几个发育阶段的表达模式。同时，我们使用亲和标签从胚胎中纯化了许多标记的蛋白质，并通过质谱法鉴定了共纯化蛋白质。这些果蝇种群可通过京都果蝇遗传资源中心公开获得。我们所有的数据都可以通过开放访问数据库（Flannotator）获得，该数据库提供了有关捕获蛋白的表达模式，亚细胞定位和体内相互作用伙伴的全面信息。我们的资源大大增加了果蝇中可用的蛋白质陷阱的数量，并确定了细胞器和结构的新标记。
Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying an artificial exon encoding yellow fluorescent protein (YFP) and protein affinity tags. From screening 41 million embryos, we recovered 616 verified independent YFP-positive lines representing protein traps in 374 genes, two-thirds of which had not been tagged in previous P element protein trap screens. Over 20 different research groups then characterized the expression patterns of the tagged proteins in a variety of tissues and at several developmental stages. In parallel, we purified many of the tagged proteins from embryos using the affinity tags and identified co-purifying proteins by mass spectrometry. The fly stocks are publicly available through the Kyoto Drosophila Genetics Resource Center. All our data are available via an open access database (Flannotator), which provides comprehensive information on the expression patterns, subcellular localisations and in vivo interaction partners of the trapped proteins. Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures.
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影响因子：
9.2
作者：
Gonsalvez, Graydon B.;Rajendra, T. K.;Matera, A. Gregory
通讯作者：
Matera, A. Gregory
影响因子：
64.8
作者：
Gavin, AC;Bösche, M;Superti-Furga, G
通讯作者：
Superti-Furga, G
影响因子：
64.8
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
通讯作者：
Celniker SE
影响因子：
16.8
作者：
Caussinus, Emmanuel;Kanca, Oguz;Affolter, Markus
通讯作者：
Affolter, Markus
影响因子：
4
作者：
Bouaouina, Mohamed;Jani, Klodiana;Calderwood, David A.
通讯作者：
Calderwood, David A.