Distribution of RGS9-2 in neurons of the mouse striatum.
Distribution of RGS9-2 in neurons of the mouse striatum.
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DOI：
10.1111/j.1471-4159.2009.06488.x
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发表时间：
2010-02
影响因子：
4.7
通讯作者：
Wensel TG
中科院分区：
文献类型：
--
作者：
Mancuso JJ;Qian Y;Long C;Wu GY;Wensel TG
Regulators of G protein Signaling (RGS) proteins negatively modulate G protein coupled receptor (GPCR) signaling activity by accelerating G protein hydrolysis of GTP, hastening pathway shutoff. A wealth of data from cell culture experiments using exogenously expressed proteins indicates that RGS9 and other RGS proteins have the potential to down-regulate a significant number of pathways. We have used an array of biochemical and tissue staining techniques to examine the subcellular localization and membrane binding characteristics of endogenous RGS9-2 and known binding partners in rodent striatum and tissue homogenates. A small fraction of RGS9-2 is present in the soluble cytoplasmic fraction, whereas the majority is present primarily associated with the plasma membrane and structures insoluble in non-ionic detergents that efficiently extract the vast majority of its binding partners, R7BP and Gβ5. It is specifically excluded from the cell nucleus in mouse striatal tissue. In cultured striatal neurons, RGS9-2 is found at extrasynaptic sites primarily along the dendritic shaft near the spine neck. Heterogeneity in RGS9-2 detergent solubility along with its unique subcellular localization suggests that its mechanism of membrane anchoring and localization is complex and likely involves additional proteins beside R7BP. An important nuclear function for RGS9-2 seems unlikely.
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影响因子：
5.3
作者：
Anderson, Garret R.;Lujan, Rafael;Martemyanov, Kirill A.
通讯作者：
Martemyanov, Kirill A.
影响因子：
64.5
作者：
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通讯作者：
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影响因子：
3.4
作者：
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通讯作者：
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DOI：
10.1016/j.bbamcr.2004.01.005
发表时间：
2004-05-03
影响因子：
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作者：
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通讯作者：
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影响因子：
16.2
作者：
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通讯作者：
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