Material Basis of the Difference between Hedysari Radix and Honey-Processed Hedysari Radix in Buzhong Yiqi.
Material Basis of the Difference between Hedysari Radix and Honey-Processed Hedysari Radix in Buzhong Yiqi.
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DOI：
10.1155/2020/4543761
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yan XK
中科院分区：
文献类型：
--
作者：
Niu JT;Cao R;Si XL;Bian TT;Xin ED;Li YF;Yan XK
比较补中益气中红芪加工前后化学成分的变化，探讨HR与蜜加工红芪(HPHR)差异的物质基础。 通过 UPLC-MS 分析 HR 和 HPHR 水提取物中的不同化合物。建立脾气虚证大鼠模型。给予大鼠不同剂量的HR或HPHR水提取物，以脾组织病理学差异、血清D-木糖、胃泌素（GAS）和淀粉酶（AMS）、白细胞介素2（IL）-2和肿瘤坏死因子-α（TNF-α）水平以及脾脏和胸腺指数为指标。研究了HR和HPHR在补中益气中功效的差异。 研究显示，与空白组相比，模型组大鼠脾组织出现脾组织损伤，主要表现为红髓与白髓界限不清，脾脏形态不规则，排列不规则，白髓结构破坏，淋巴细胞减少，生发中心数量减少或萎缩。与模型组比较，HR、HPHR中、高剂量组对脾气虚大鼠脾组织有保护作用，且HPHR作用更强；与模型组比较,各治疗组大鼠血清D-木糖、GAS、AMS水平以及胸腺、脾指数均显着升高,血清IL-2、TNF-α水平显着降低,其中HPHR组的调节作用优于HR组。 HR和HPHR的水提取物中共发现16种差异化合物，其中HPHR中的10种化合物较HR上调，而6种化合物较HR下调。 结果表明，HR和HPHR均能改善大鼠脾气虚证，且后者药效优于前者。 HR 和 HPHR 组成部分的差异可能会导致功效的变化。
To compare the changes of chemical components of Hedysari Radix (HR) before and after honey-processing, and to explore the material basis of the difference between HR and honey-processed Hedysari Radix (HPHR) in Buzhong Yiqi. Different compounds in aqueous extracts of HR and HPHR were analysed by UPLC-MS. A rat model of spleen qi deficiency was established. The rats were treated with different doses of water extracts of HR or HPHR, and pathological differences in spleen tissue, serum levels of D-xylose, gastrin (GAS) and amylase (AMS) interleukin-2 (IL)-2 and tumour necrosis factor-α (TNF-α), as well as spleen and thymus indices, were used as indicators. Differences in the efficacy of HR and HPHR in Buzhong Yiqi were studied. The research showed that compared with the blank group, the spleen tissue of rats in the model group showed spleen tissue damage, which mainly manifested as unclear boundaries between red pulp and white pulp, irregular spleen morphology and irregular arrangement, and the structure of white pulp destruction, less lymphocytes, the number of germinal centers decreased or atrophied. Compared with the model group, the middle and high dose groups of HR and HPHR had protective effects on spleen tissue of spleen-qi deficiency rats, and HPHR had a stronger effect; compared with those in the model group, rats in each treatment group showed remarkably higher serum D-xylose, GAS and AMS levels and thymus and spleen indices, and remarkably lower serum IL-2 and TNF-α levels, among which HPHR group showed better regulation effect than HR group. A total of 16 differential compounds were found in the aqueous extracts of HR and HPHR, of which 10 compounds in HPHR were up regulated, while 6 compounds were down regulated compare to HR. The results indicated that both HR and HPHR can improve spleen qi deficiency syndrome of rats, the pharmacodynamic effect of the latter was better than the former. Differences in components of HR and HPHR potentially leading to variations in efficacy.
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影响因子：
--
作者：
Zhu Ling-xia;Sun Xiao-yan;Cao Peng
通讯作者：
Cao Peng
DOI：
10.19540/j.cnki.cjcmm.20190916.301
发表时间：
2019-12-01
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
Zhong, Ling-Yun;Cui, Mei-Na;Gong, Qian-Feng
通讯作者：
Gong, Qian-Feng
影响因子：
5.4
作者：
Zheng, Xiao-fen;Tian, Jun-sheng;Qin, Xue-mei
通讯作者：
Qin, Xue-mei
中医学组学促进四君子汤治疗脾气虚证的质量标志物发现
影响因子：
8.1
作者：
Zhao，Qiqi;Gao，Xin;Wang，Xijun
通讯作者：
Wang，Xijun