Competitive control of endoglucanase gene engXCA expression in the plant pathogen Xanthomonas campestris by the global transcriptional regulators HpaR1 and Clp.
Competitive control of endoglucanase gene engXCA expression in the plant pathogen Xanthomonas campestris by the global transcriptional regulators HpaR1 and Clp.
复制标题
全局转录调节因子 HpaR1 和 Clp 竞争性控制植物病原体黄单胞菌中内切葡聚糖酶基因 engXCA 的表达
DOI：
10.1111/mpp.12739
复制
发表时间：
2019-01
影响因子：
4.9
通讯作者：
Tang JL
中科院分区：
文献类型：
--
作者：
Liu GF;Su HZ;Sun HY;Lu GT;Tang JL
SUMMARY Transcriptional regulators are key players in pathways that allow bacteria to alter gene expression in response to environmental conditions. However, work to understand how such transcriptional regulatory networks interact in bacterial plant pathogens is limited. Here, in the phytopathogen Xanthomonas campestris, we demonstrate that the global transcriptional regulator HpaR1 influences many of the same genes as another global regulator Clp, including the engXCA gene that encodes extracellular endoglucanase. We demonstrate that HpaR1 facilitates the binding of RNA polymerase to the engXCA promoter. In addition, we show that HpaR1 binds directly to the engXCA promoter. Furthermore, our in vitro tests characterize two binding sites for Clp within the engXCA promoter. Interestingly, one of these sites overlaps with the HpaR1 binding site. Mobility shift assays reveal that HpaR1 has greater affinity for binding to the engXCA promoter. This observation is supported by promoter activity assays, which show that the engXCA expression level is lower when both HpaR1 and Clp are present together, rather than alone. The data also reveal that HpaR1 and Clp activate engXCA gene expression by binding directly to its promoter. This transcriptional activation is modulated as both regulators compete to bind to overlapping sites on the engXCA promoter. Bioinformatics analysis suggests that this mechanism may be used broadly in Xanthomonas campestris pv. campestris (Xcc) and is probably widespread in Xanthomonads and, potentially, other bacteria. Taken together, these data support a novel mechanism of competitive activation by two global regulators of virulence gene expression in Xcc which is probably widespread in Xanthomonads and, potentially, other bacteria.
登录
查看更多内容
影响因子：
3.2
作者：
DECRECYLAGARD, V;GLASER, P;DANCHIN, A
通讯作者：
DANCHIN, A
影响因子：
4.6
作者：
Su HZ;Wu L;Qi YH;Liu GF;Lu GT;Tang JL
通讯作者：
Tang JL
影响因子：
5.1
作者：
Li, Rui-Fang;Lu, Guang-Tao;Tang, Ji-Liang
通讯作者：
Tang, Ji-Liang
影响因子：
3.2
作者：
Santos-Beneit, Fernando;Rodriguez-Garcia, Antonio;Martin, Juan F.
通讯作者：
Martin, Juan F.
影响因子：
6.7
作者：
Ryan RP;An SQ;Allan JH;McCarthy Y;Dow JM
通讯作者：
Dow JM