Effects of low temperature plasmas and plasma activated waters on Arabidopsis thaliana germination and growth.
Effects of low temperature plasmas and plasma activated waters on Arabidopsis thaliana germination and growth.
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DOI：
10.1371/journal.pone.0195512
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Yousfi M
中科院分区：
文献类型：
--
作者：
Bafoil M;Jemmat A;Martinez Y;Merbahi N;Eichwald O;Dunand C;Yousfi M
利用两种大气压等离子体装置（空气介质阻挡放电和氦等离子体射流）对拟南芥种子萌发初期的萌发过程进行了研究。然后，在植物发育和生长的后期使用等离子体活化的沃茨，直到42天。在萌发阶段对种皮和胚乳破裂的影响是显着的，在空气等离子体的情况下，由于其更高的能量和效率比氦等离子体的情况下产生活性氧。后者仅对种皮破裂有明显的影响。发芽刺激的分析是基于活性氧产生的特异性染色，过氧化物酶活性和膜渗透性试验。此外，扫描电子显微镜（SEM）显示，空气等离子体处理的种子表面更光滑，可以解释等离子体诱导发芽。在生长阶段期间，使用4种水（自来水和去离子沃茨，通过低温等离子体射流活化或未活化）对植物进行浇水。关于其他水种类，自来水的表征显示出更大的电导率、酸度和活性氮和氧物质的浓度。只有被等离子体射流激活的自来水对植物生长显示出显著的影响。这种效应可能与活性氮物质如等离子体活化的自来水中存在的亚硝酸盐/硝酸盐物质相关。
Two plasma devices at atmospheric pressure (air dielectric barrier discharge and helium plasma jet) have been used to study the early germination of Arabidopsis thaliana seeds during the first days. Then, plasma activated waters are used during the later stage of plant development and growth until 42 days. The effects on both testa and endospserm ruptures during the germination stage are significant in the case of air plasma due to its higher energy and efficiency of producing reactive oxygen species than the case of helium plasma. The latter has shown distinct effects only for testa rupture. Analysis of germination stimulations are based on specific stainings for reactive oxygen species production, peroxidase activity and also membrane permeability tests. Furthermore, scanning electron microscopy (SEM) has shown a smoother seed surface for air plasma treated seeds that can explain the plasma induced-germination. During the growth stage, plants were watered using 4 kinds of water (tap and deionized waters activated or not by the low temperature plasma jet). With regards to other water kinds, the characterization of the tap water has shown a larger conductivity, acidity and concentration of reactive nitrogen and oxygen species. Only the tap water activated by the plasma jet has shown a significant effect on the plant growth. This effect could be correlated to reactive nitrogen species such as nitrite/nitrate species present in plasma activated tap water.
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