An antimicrobial protein of the gut symbiont Bacteroides fragilis with a MACPF domain of host immune proteins.
An antimicrobial protein of the gut symbiont Bacteroides fragilis with a MACPF domain of host immune proteins.
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肠道共生型细菌的抗菌蛋白具有宿主免疫蛋白的MACPF结构域。
DOI：
10.1111/mmi.12839
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发表时间：
2014-12
影响因子：
3.6
通讯作者：
Comstock LE
中科院分区：
文献类型：
--
作者：
Chatzidaki-Livanis M;Coyne MJ;Comstock LE
拟杆菌属是人类肠道微生物群中数量最多的革兰氏阴性菌，在许多个体中占细菌总数的一半以上。这些细菌用来在这个生态系统中建立和维持自己的一些因素已经开始被确定。然而，生态竞争，特别是一种生物直接伤害另一种生物的干扰竞争，在很大程度上尚未被探索。为了开始理解这一生态原理的相关性，因为它适用于这些丰富的肠道细菌和可能促进这种竞争的因素，我们筛选了脆弱拟杆菌来生产抗菌分子。我们发现细胞外分泌抗菌分子的生产在这个物种中很普遍。本文中描述的第一个确定的分子包含一个膜攻击复合物/穿孔素（MACPF）结构域，该结构域存在于宿主免疫分子中，通过孔形成杀死细菌和病毒感染的细胞，影响该结构域关键残基的突变使其活性丧失。这种抗菌分子被称为BSAP-1，从细胞的外膜囊泡中分泌出来，它的分泌、加工或产生细胞的免疫不需要额外的蛋白质。这项研究提供了对分泌分子的第一次洞察，这些分子促进了人类肠道杆菌菌株之间的竞争干扰。
Bacteroidales are the most abundant Gram negative bacteria of the human intestinal microbiota comprising more than half of the bacteria in many individuals. Some of the factors that these bacteria use to establish and maintain themselves in this ecosystem are beginning to be identified. However, ecological competition, especially interference competition where one organism directly harms another, is largely unexplored. To begin to understand the relevance of this ecological principle as it applies to these abundant gut bacteria and factors that may promote such competition, we screened Bacteroides fragilis for the production of antimicrobial molecules. We found that the production of extracellularly secreted antimicrobial molecules is widespread in this species. The first identified molecule, described in this manuscript, contains a membrane attack complex/perforin (MACPF) domain present in host immune molecules that kill bacteria and virally infected cells by pore formation, and mutations affecting key residues of this domain abrogated its activity. This antimicrobial molecule, termed BSAP-1, is secreted from the cell in outer membrane vesicles and no additional proteins are required for its secretion, processing or immunity of the producing cell. This study provides the first insight into secreted molecules that promote competitive interference among Bacteroidales strains of the human gut.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
6.4
作者：
Coyne MJ;Zitomersky NL;McGuire AM;Earl AM;Comstock LE
通讯作者：
Comstock LE
影响因子：
6.4
作者：
Elhenawy W;Debelyy MO;Feldman MF
通讯作者：
Feldman MF
影响因子：
64.8
作者：
Kirkup, BC;Riley, MA
通讯作者：
Riley, MA
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer