Lipolysis in Brown Adipocytes Is Not Essential for Cold-Induced Thermogenesis in Mice.
Lipolysis in Brown Adipocytes Is Not Essential for Cold-Induced Thermogenesis in Mice.
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DOI：
10.1016/j.cmet.2017.09.002
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发表时间：
2017-11-07
期刊：
影响因子：
29
通讯作者：
Yu L
中科院分区：
文献类型：
--
作者：
Shin H;Ma Y;Chanturiya T;Cao Q;Wang Y;Kadegowda AKG;Jackson R;Rumore D;Xue B;Shi H;Gavrilova O;Yu L
棕色脂肪组织（BAT）中的脂滴（LD）脂解通常被认为是冷诱导的非颤抖性产热所必需的。在这里，我们表明，小鼠缺乏BAT比较基因鉴定-58（CGI-58），一个脂解激活剂刺激LD脂解必不可少的，有正常的产热能力，是不冷敏感。相对于同窝对照组，这些动物在冷暴露期间提供食物时体温较高。在喂食、冷暴露的基因敲除小鼠中，体温升高与能量消耗增加、交感神经支配增加和白色脂肪组织（WAT）的布朗宁相关。在BAT和WAT中都缺乏CGI-58的小鼠是冷敏感的，但仅在禁食状态下。因此，BAT中的LD脂解对于体内冷诱导的非颤抖性产热不是必需的。相反，BAT中的CGI-58依赖性LD脂解调节WAT产热，我们的数据揭示了WAT脂解在禁食期间促进产热中的重要作用。棕色脂肪中的脂解通常被认为是冷诱导产热所必需的。Shin等人表明，棕色脂肪分解缺陷的小鼠对冷不敏感。这些动物通过增加白色脂肪布朗宁和来自饮食或白色脂肪脂解的燃料燃烧来弥补缺陷。
Lipid droplet (LD) lipolysis in brown adipose tissue (BAT) is generally considered to be required for cold-induced nonshivering thermogenesis. Here we show that mice lacking BAT Comparative Gene Identification-58 (CGI-58), a lipolytic activator essential for the stimulated LD lipolysis, have normal thermogenic capacity and are not cold sensitive. Relative to littermate controls, these animals had higher body temperatures when they were provided food during cold exposure. The increase in body temperature in the fed, cold-exposed knockout mice was associated with increased energy expenditure and increased sympathetic innervation and browning of white adipose tissue (WAT). Mice lacking CGI-58 in both BAT and WAT were cold sensitive, but only in the fasted state. Thus, LD lipolysis in BAT is not essential for cold-induced nonshivering thermogenesis in vivo. Rather CGI-58-dependent LD lipolysis in BAT regulates WAT thermogenesis, and our data uncover an essential role of WAT lipolysis in fueling thermogenesis during fasting. Lipolysis in brown fat is generally considered to be required for cold-induced thermogenesis. Shin et al. show that mice defective in brown fat lipolysis are not cold sensitive. These animals compensate for the defect by increasing white fat browning and combustion of fuels derived from diet or white fat lipolysis.
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