Cesium‐insensitive mutants of Arabidopsis thaliana
Cesium‐insensitive mutants of Arabidopsis thaliana
复制标题
拟南芥铯不敏感突变体
DOI：
--
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
M. Sussman
中科院分区：
文献类型：
--
作者：
J. Sheahan;Leonidio Ribeiro;M. Sussman
总结
在动物和微生物中，溶质跨质膜转运的分子分析得到了明确的转运突变体分析的帮助。为了获得阳离子转运遗传缺陷的突变体植物，测试了一价阳离子（Li+、Na+、Rb+和Cs+）对拟南芥种子萌发的抑制作用。铯的独特之处在于，在低浓度下，它强烈抑制幼苗发育。本文证明了铯对A.拟南芥及其毒性与钾供应水平密切相关。获得的条件，以最大限度地提高铯的敏感性，种子发芽在一个大的人口，和选择抗性使用M2种子来自甲基磺酸乙酯（EMS）处理的植物产生了几十个抗性植物。来自这些植物的种子产生铯不敏感的突变株系与能量依赖性钾吸收的遗传变化。在从回交到野生型植物的后代中，至少一个品系显示出单基因隐性突变所预期的分离比，并且RFLP分析将突变基因座定位到染色体4的顶部。
Summary
The molecular analysis of solute transport across the plasma membrane in animals and microorganisms has been aided by the analysis of well-defined transport mutants. To obtain mutant plants with genetic defects in cation transport, the inhibitory effect of monovalent cations (Li+, Na+, Rb+, and Cs+) on Arabidopsis thaliana seed germination was tested. Cesium was unique in that at low concentrations it strongly inhibited seedling development. In this report it is demonstrated that cesium is a competitive inhibitor for potassium transport in A. thaliana and its toxicity is closely tied to the level of potassium supplied. Conditions were obtained to maximize the cesium-sensitivity for seed germination in a large population, and selection for resistance using M2 seeds derived from ethyl methane sulfonate (EMS)-treated plants yielded several dozen resistant plants. Seeds derived from these plants yielded cesium-Insensitive mutant lines with heritable changes in energy-dependent potassium uptake. In progeny from a backcross to wild-type plants, at least one of the lines showed the segregation ratio expected for a single-gene recessive mutation and an RFLP analysis mapped the mutant locus to the top of chromosome 4.
推导的人羧肽酶 N 高分子量亚基的蛋白质序列揭示了富含亮氨酸的串联重复序列的存在。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tan,F;Weerasinghe,DK;Skidgel,RA;Tamei,H;Kaul,RK;Roninson,IB;Schilling,JW;Erdös,EG
通讯作者：
Erdös,EG
DOI：
10.1073/pnas.85.18.6856
发表时间：
1988-09-01
影响因子：
11.1
作者：
CHANG, C;BOWMAN, JL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM