Distribution of bone-marrow-derived endothelial and immune cells in a murine colitis-associated colorectal cancer model.
Distribution of bone-marrow-derived endothelial and immune cells in a murine colitis-associated colorectal cancer model.
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小鼠结肠炎相关结直肠癌模型中骨髓源性内皮细胞和免疫细胞的分布
DOI：
10.1371/journal.pone.0073666
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Guleng B
中科院分区：
文献类型：
--
作者：
Xiao CX;Wang HH;Shi Y;Li P;Liu YP;Ren JL;Guleng B
炎症性肠病（IBD）可导致患结直肠癌（CRC）的风险增加。本研究的目的是建立联合骨髓移植（BMT）和结肠炎相关结直肠癌（CAC）的模型，并确定BM来源的细胞在与癌变相关的炎症过程中的贡献。我们使用绿色荧光蛋白（GFP）转基因小鼠建立了BMT模型，然后用AOM/DSS诱导CAC，并对活体组织和冷冻肿瘤切片进行了共聚焦显微镜分析。我们的成像分析显示，GFP阳性细胞广泛浸润的肿瘤间质和一些WGA和GFP或CD 31和GFP双阳性细胞中观察到的肿瘤血管衬里。流式细胞术分析肿瘤浸润细胞显示，GFP阳性的CD 11 c + DCs细胞是GFP+/CD 11 C-细胞的三分之一，并且这些DCs中的一半（0.96% vs 1.02%）是GFP阳性的BM衍生细胞。大部分CD 4 + T细胞为GFP阴性（12.02%vs1.9%），我们发现了一个新的CD 4 + CD 11 c + DC亚群（0.34%vs1.64%）。总之，我们确定了BM源性内皮细胞，CD 11 c + DC和CD 4 + T细胞在肿瘤中的分布。该模型可能有助于阐明结肠炎相关结直肠癌进展过程中不同的BM衍生细胞类型和功能。
Inflammatory bowel disease (IBD) can lead to an increased risk of developing colorectal cancer (CRC). The aim of this study was to establish a model for combined bone marrow transplantation (BMT) and colitis-associated colorectal cancer (CAC) and to define the contribution of BM-derived cells during the inflammation associated with carcinogenesis. We established a model for BMT using green fluorescent protein (GFP) transgenic mice, followed by AOM/DSS-induced CAC, and performed confocal microscopy analysis on in vivo living tissue and frozen tumor sections. Our imaging analyses showed that GFP-positive cells extensively infiltrated the tumor stroma and that some WGA and GFP or CD31 and GFP double-positive cells were observed in the lining of tumor vessels. Flow cytometry analysis of the tumor-infiltrating cells showed that the GFP-positive CD11c+ DCs cells were one-third of the GFP+/CD11C- cells, and that half of these DCs (0.96% vs 1.02%) were GFP-positive BM-derived cells. The majority of CD4+ T cells were GFP-negative (12.02% vs 1.9%), and we discovered a novel CD4+ CD11c+ DC subset (0.34% vs 1.64%). In conclusion, we defined the distribution of BM-derived endothelial cells, CD11c+ DCs and CD4+ T cells in tumors. This model might be useful for elucidating the diverse BM-derived cell types and functions during the progression of colitis-associated colorectal cancer.
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影响因子：
0.7
作者：
Laurent, Julien;Touvrey, Cedric;Ruegg, Curzio
通讯作者：
Ruegg, Curzio
影响因子：
11.2
作者：
Guleng, B;Tateishi, K;Omata, M
通讯作者：
Omata, M
影响因子：
3.2
作者：
Ferrara, Napoleone
通讯作者：
Ferrara, Napoleone
影响因子：
82.9
作者：
Okamoto, R;Yajima, T;Watanabe, M
通讯作者：
Watanabe, M
影响因子：
4.4
作者：
Brinster, C;Shevach, EM
通讯作者：
Shevach, EM