Synthetic polymer coatings diminish chronic inflammation risk in large ECM-based materials.
Synthetic polymer coatings diminish chronic inflammation risk in large ECM-based materials.
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DOI：
10.1002/jbm.a.36564
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发表时间：
2019-03
影响因子：
4.9
通讯作者：
Fisher, John P.
中科院分区：
文献类型：
--
作者：
Bracaglia, Laura G.;Winston, Shira;Powell, Douglas A.;Fisher, John P.
支持细胞浸润和重塑的可降解材料是血管修复的组织工程方法的基础。此外，为了替换或闭合大面积的脉管系统，补片材料或支架还必须随时间经受高压。基于细胞外基质（ECM）的支架提供了一种具有环境线索的生物基质，可以支持适当血管组织的形成。然而，由纯天然材料制成的支架会迅速降解，导致植入物的机械完整性降低，并可能导致该部位的慢性炎症。将基质致密组织心包与可降解聚合物聚（富马酸丙二醇酯）（PPF）层组合的混合生物材料适合于承受快速酶促降解并控制未改变的天然组织基质的呈现以进行重塑活性。在这项研究中，我们表明，聚合物增强的混合物支持细胞浸润，但在无胸腺和免疫活性大鼠模型中，6周后体内植入物附近的巨噬细胞比未处理的组织对照少。这一结果得到了其他炎症细胞群中观察到的变化的支持。基于对未处理的心包和PPF增强的心包的炎症反应的显著差异，我们得出结论，聚合物增强层可以用作在基于ECM的支架中利用ECM分子的呈现的工具。
Degradable materials that can support cell infiltration and remodeling are the basis of tissue engineered approaches to vascular repair. In addition, to replace or close a large area of the vasculature, a patch material or scaffold must also withstand high pressure over time. Extracellular matrix-based (ECM-based) scaffolds offer a biological substrate with environmental cues that can support the formation of appropriate vascular tissue. However, scaffolds made from pure natural materials can degrade rapidly, resulting in reduced mechanical integrity of the implant and possible chronic inflammation in the site. A hybrid biomaterial, combining the matrix-dense tissue pericardium with a layer of the degradable polymer poly(propylene fumarate) (PPF), is suited to withstand rapid enzymatic degradation and control the presentation of an unaltered natural tissue matrix for remodeling activity. In this study, we show that the polymer reinforced hybrid supports cellular infiltration, but has fewer macrophages in the vicinity of the implant after 6 weeks in vivo than an untreated tissue control in both athymic and immunocompetent rat models. This result is supported by changes seen in other inflammatory cell populations. Based on significant differences in the inflammatory response to untreated pericardium and PPF-reinforced pericardium, we conclude that the polymer reinforcement layer can be used as a tool to leverage presentation of the ECM molecules in ECM-based scaffolds.
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影响因子：
3.8
作者：
Balestrini, Jenna L.;Niklason, Laura E.
通讯作者：
Niklason, Laura E.
DOI：
10.1016/j.jtcvs.2009.09.057
发表时间：
2010-02-01
影响因子：
6
作者：
Hibino, Narutoshi;McGillicuddy, Edward;Shinoka, Toshiharu
通讯作者：
Shinoka, Toshiharu
影响因子：
4.8
作者：
Hibino, Narutoshi;Mejias, Dane;Breuer, Christopher
通讯作者：
Breuer, Christopher
影响因子：
6.1
作者：
Bellon, Teresa;Martinez, Virginia;Auxiliadora Bajo, Maria
通讯作者：
Auxiliadora Bajo, Maria
影响因子：
2.8
作者：
Gaspardone, A;Crea, F;Gioffrè, PA
通讯作者：
Gioffrè, PA