Genomic comparison of Escherichia coli O104:H4 isolates from 2009 and 2011 reveals plasmid, and prophage heterogeneity, including shiga toxin encoding phage stx2.
Genomic comparison of Escherichia coli O104:H4 isolates from 2009 and 2011 reveals plasmid, and prophage heterogeneity, including shiga toxin encoding phage stx2.
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DOI：
10.1371/journal.pone.0048228
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Threat Characterization Consortium
中科院分区：
文献类型：
--
作者：
Ahmed SA;Awosika J;Baldwin C;Bishop-Lilly KA;Biswas B;Broomall S;Chain PS;Chertkov O;Chokoshvili O;Coyne S;Davenport K;Detter JC;Dorman W;Erkkila TH;Folster JP;Frey KG;George M;Gleasner C;Henry M;Hill KK;Hubbard K;Insalaco J;Johnson S;Kitzmiller A;Krepps M;Lo CC;Luu T;McNew LA;Minogue T;Munk CA;Osborne B;Patel M;Reitenga KG;Rosenzweig CN;Shea A;Shen X;Strockbine N;Tarr C;Teshima H;van Gieson E;Verratti K;Wolcott M;Xie G;Sozhamannan S;Gibbons HS;Threat Characterization Consortium
2011年5月，一种肠聚集性大肠杆菌O104:H4菌株获得了一种志贺毒素2转化噬菌体，在欧洲引起了大规模的血性腹泻暴发，其中以溶血性尿毒症综合征病例的高发而闻名。有几项研究描述了与此次暴发有关的菌株的基因组清单和系统发育，并使用基因组草图汇编收集了历史上的大肠杆菌O104:H4分离株。我们提供了2011年暴发（2011C-3493）分离株和2009年格鲁吉亚共和国发生的出血性腹泻病例分离株（2009EL-2050和2009EL-2071）的完整、封闭基因组序列。比较基因组分析表明，虽然格鲁吉亚菌株是迄今测序的2011年爆发分离株的最近邻居，但在Stx2噬菌体基因组、mer/tet抗生素耐药岛以及菌株的前噬菌体和质粒谱（包括先前未描述的与鼠疫耶尔森氏菌pMT毒力质粒同源的质粒）中，结构和核苷酸水平差异很明显。此外，多表型分析表明，2009EL-2071对多粘菌素和膜破坏剂具有较高的抗性。最后，我们通过电子显微镜显示了欧洲和格鲁吉亚菌株之间存在共同噬菌体形态型的证据，格鲁吉亚菌株之间存在第二种噬菌体形态型。宿主遗传背景中存在至少两种stx2噬菌体基因型，可能源于2011年暴发分离株的最近共同祖先，这表明含有stx2噬菌体的大肠杆菌O104:H4菌株的出现可能不止一次，或者当前暴发的分离株可能是最近将新的stx2噬菌体元件转移到先前存在的stx2阳性遗传背景的结果。
In May of 2011, an enteroaggregative Escherichia coli O104:H4 strain that had acquired a Shiga toxin 2-converting phage caused a large outbreak of bloody diarrhea in Europe which was notable for its high prevalence of hemolytic uremic syndrome cases. Several studies have described the genomic inventory and phylogenies of strains associated with the outbreak and a collection of historical E. coli O104:H4 isolates using draft genome assemblies. We present the complete, closed genome sequences of an isolate from the 2011 outbreak (2011C–3493) and two isolates from cases of bloody diarrhea that occurred in the Republic of Georgia in 2009 (2009EL–2050 and 2009EL–2071). Comparative genome analysis indicates that, while the Georgian strains are the nearest neighbors to the 2011 outbreak isolates sequenced to date, structural and nucleotide-level differences are evident in the Stx2 phage genomes, the mer/tet antibiotic resistance island, and in the prophage and plasmid profiles of the strains, including a previously undescribed plasmid with homology to the pMT virulence plasmid of Yersinia pestis. In addition, multiphenotype analysis showed that 2009EL–2071 possessed higher resistance to polymyxin and membrane-disrupting agents. Finally, we show evidence by electron microscopy of the presence of a common phage morphotype among the European and Georgian strains and a second phage morphotype among the Georgian strains. The presence of at least two stx2 phage genotypes in host genetic backgrounds that may derive from a recent common ancestor of the 2011 outbreak isolates indicates that the emergence of stx2 phage-containing E. coli O104:H4 strains probably occurred more than once, or that the current outbreak isolates may be the result of a recent transfer of a new stx2 phage element into a pre-existing stx2-positive genetic background.
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影响因子：
3
作者：
Andreoli, SP;Trachtman, H;Obrig, TG
通讯作者：
Obrig, TG
影响因子：
3
作者：
Chang, Wenling E.;Sarver, Keri;Sozhamannan, Shanmuga
通讯作者：
Sozhamannan, Shanmuga
DOI：
10.1126/science.1180614
发表时间：
2009-10-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chain PS;Grafham DV;Fulton RS;Fitzgerald MG;Hostetler J;Muzny D;Ali J;Birren B;Bruce DC;Buhay C;Cole JR;Ding Y;Dugan S;Field D;Garrity GM;Gibbs R;Graves T;Han CS;Harrison SH;Highlander S;Hugenholtz P;Khouri HM;Kodira CD;Kolker E;Kyrpides NC;Lang D;Lapidus A;Malfatti SA;Markowitz V;Metha T;Nelson KE;Parkhill J;Pitluck S;Qin X;Read TD;Schmutz J;Sozhamannan S;Sterk P;Strausberg RL;Sutton G;Thomson NR;Tiedje JM;Weinstock G;Wollam A;Genomic Standards Consortium Human Microbiome Project Jumpstart Consortium;Detter JC
通讯作者：
Detter JC
影响因子：
3.1
作者：
Czeczulin, JR;Balepur, S;Nataro, JP
通讯作者：
Nataro, JP
影响因子：
4.4
作者：
Aziz RK;Bartels D;Best AA;DeJongh M;Disz T;Edwards RA;Formsma K;Gerdes S;Glass EM;Kubal M;Meyer F;Olsen GJ;Olson R;Osterman AL;Overbeek RA;McNeil LK;Paarmann D;Paczian T;Parrello B;Pusch GD;Reich C;Stevens R;Vassieva O;Vonstein V;Wilke A;Zagnitko O
通讯作者：
Zagnitko O