Red Blood Cell Responses during a Long-Standing Load in a Microfluidic Constriction
Red Blood Cell Responses during a Long-Standing Load in a Microfluidic Constriction
复制标题
在微流体收缩中长期负载期间的红细胞反应
DOI：
10.3390/mi8040100
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发表时间：
2017-03-26
期刊：
影响因子：
3.4
通讯作者：
Kaneko M
中科院分区：
文献类型：
--
作者：
Horade M;Tsai CH;Ito H;Kaneko M
通过实验研究了长期负荷期间红细胞的反应。通过高速相机和高速执行器，我们能够操纵微流体收缩内的细胞，并且每个细胞由于几何限制而被压缩。在收缩内部的负载过程中，发现细胞的颜色逐渐变暗，而细胞的长度变得越来越短。根据5分钟负载的分析结果，在8位颜色分辨率下，单元暗度平均增加60.9，单元长度平均收缩为初始长度的15%。从一个细胞到另一个细胞，一致地观察到相同的趋势。根据实验结果建立了颜色变化和长度之间的相关性。这些变化被认为部分是由于细胞的粘弹性特性，即细胞的结构随着时间的推移而变化，以适应收缩内的有限空间。
Red blood cell responses during a long-standing load were experimentally investigated. With a high-speed camera and a high-speed actuator, we were able to manipulate cells staying inside a microfluidic constriction, and each cell was compressed due to the geometric constraints. During the load inside the constriction, the color of the cells was found to gradually darken, while the cell lengths became shorter and shorter. According to the analysis results of a 5 min load, the average increase of the cell darkness was 60.9 in 8-bit color resolution, and the average shrinkage of the cell length was 15% of the initial length. The same tendency was consistently observed from cell to cell. A correlation between the changes of the color and the length were established based on the experimental results. The changes are believed partially due to the viscoelastic properties of the cells that the cells’ configurations change with time for adapting to the confined space inside the constriction.
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影响因子：
4.6
作者：
Ito H;Murakami R;Sakuma S;Tsai CD;Gutsmann T;Brandenburg K;Pöschl JM;Arai F;Kaneko M;Tanaka M
通讯作者：
Tanaka M
影响因子：
3.4
作者：
MARKLE, DR;EVANS, EA;HOCHMUTH, RM
通讯作者：
HOCHMUTH, RM
影响因子：
6.1
作者：
Roth KB;Eggleton CD;Neeves KB;Marr DW
通讯作者：
Marr DW
DOI：
10.1073/pnas.1606751113
发表时间：
2016-07-12
影响因子：
11.1
作者：
Pivkin, Igor V.;Peng, Zhangli;Suresh, Subra
通讯作者：
Suresh, Subra
影响因子：
3.2
作者：
Tomaiuolo, Giovanna
通讯作者：
Tomaiuolo, Giovanna