NO reactions with sol-gel and solution phase samples of the ferric nitrite derivative of HbA.
NO reactions with sol-gel and solution phase samples of the ferric nitrite derivative of HbA.
复制标题
DOI：
10.1016/j.niox.2009.11.003
复制
发表时间：
2010-02-15
期刊：
影响因子：
--
通讯作者：
Friedman JM
中科院分区：
文献类型：
--
作者：
Roche CJ;Friedman JM
一氧化氮（NO）与人成人血红蛋白Hb的亚硝酸铁（Met）衍生物的反应被探测为溶液相和溶胶-凝胶包封的群体。的Q带吸收光谱和拟合的Hb衍生物的人口的演变被用来显示发生的事件的顺序时，NO与亚硝酸盐结合到血红蛋白中的铁血红素相互作用。溶胶-凝胶是用来比较不断变化的人口作为一个功能的四元状态的起始亚硝酸盐人口。使用CN−阴离子探测中间体的氧化还原状态以捕获铁血红素物种。反应过程中出现的反应性NO物种，如N2 O3的存在下，使用荧光探针的探测-2，而荧光团Chemi-fluor被用作间接测量的反应的能力，以创建S-亚硝基硫醇谷胱甘肽。结果是一致的形成一个稳定的反应中间体能够产生生物活性形式的NO。观察到的模式是一致的，从而NO与亚硝酸铁衍生物反应生成N2 O3的建议机制。
The reaction of nitric oxide (NO) with the ferric (met) nitrite derivative of human adult hemoglobin Hb is probed for both solution phase and sol-gel encapsulated populations. The evolution of both the Q band absorption spectrum and fitted populations of Hb derivatives are used to show the sequence of events occurring when NO interacts with nitrite bound to a ferric heme in Hb. The sol-gel is used to compare the evolving populations as a function of quaternary state for the starting met nitrite populations. The redox status of intermediates is probed using the CN− anion to trap ferric heme species. The emergent presence of reactive NO species such as N2O3 during the course of the reaction is probed using the fluorescent probe DAF-2 whereas the fluorophore Chemi-fluor is used as an indirect measure of the ability of the reaction to create S-nitrosothiols on glutathione. The results are consistent with the formation of a stable reactive intermediate capable of generating bioactive forms of NO. The patterns observed are consistent with a proposed mechanism whereby NO reacts with the ferric nitrite derivative to generate N2O3.
登录
查看更多内容
影响因子：
3.9
作者：
ARNELLE, DR;STAMLER, JS
通讯作者：
STAMLER, JS
影响因子：
--
作者：
Abbruzzetti, S;Viappiani, C;Mozzarelli, A
通讯作者：
Mozzarelli, A
DOI：
10.1016/j.jamcollsurg.2004.07.025
发表时间：
2004-11-01
影响因子：
5.2
作者：
Fitzpatrick, CM;Savage, SA;Kashyap, VS
通讯作者：
Kashyap, VS
DOI：
10.1152/ajpheart.00146.2004
发表时间：
2004-10-01
影响因子：
4.8
作者：
Cabrales, P;Kanika, ND;Intaglietta, M
通讯作者：
Intaglietta, M
影响因子：
8
作者：
Bruno, S;Bonaccio, M;Mozzarelli, A
通讯作者：
Mozzarelli, A