Deep Sequencing of Small RNA Reveals the Molecular Regulatory Network of AtENO2 Regulating Seed Germination.
Deep Sequencing of Small RNA Reveals the Molecular Regulatory Network of AtENO2 Regulating Seed Germination.
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小RNA深度测序揭示AtENO2调节种子萌发的分子调控网络
DOI：
10.3390/ijms22105088
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发表时间：
2021-05-11
影响因子：
5.6
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Wu Y;Zheng L;Bing J;Liu H;Zhang G
种子萌发是植物新生命周期中的关键步骤。在农业上，我们把种子快速而稳定的萌发过程作为衡量作物优质和产量的必要条件之一。ENO2是糖酵解的关键酶，在植物生长和非生物胁迫反应中也起着重要作用。在我们的研究中，我们发现AtenO2突变体(eno2−)的种子萌发时间早于野生型(WT)。以往的研究表明，microRNAs(MiRNAs)在种子萌发过程中起着至关重要的作用。对小RNA进行深度测序后，共发现590个差异表达的miRNAs，其中87个差异表达miRNAs。通过对miRNAs靶基因的预测和GO注释的分析，我们发现了18个与种子萌发相关的基因，包括ARF家族、TIR1、INVC、RR19、TUDOR2、GA3OX2、PXMT1和TGA1。MiR9736-z、miR5059-z、ath-miR167a-5p、ath-miR167b、ath-miR5665、ath-miR866-3p、miR10186-z、miR8165-z、ath-miR857、ath-miR399b、ath-miR399c-3p、miR399-y、miR163-z、ath-miR393a-5p和ath-miR393b-5p是调控种子萌发相关基因的关键miRNAs。通过KEGG富集分析，我们发现植物激素信号转导途径显著丰富，这些miRNAs也参与了植物激素信号转导途径基因的调控，从而影响植物激素的合成，进而影响种子萌发过程。本研究为进一步探索AtENO2对种子萌发的调控奠定了基础。
Seed germination is a key step in the new life cycle of plants. In agriculture, we regard the rapid and consistent process of seed germination as one of the necessary conditions to measure the high quality and yield of crops. ENO2 is a key enzyme in glycolysis, which also plays an important role in plant growth and abiotic stress responses. In our study, we found that the time of seed germination in AtENO2 mutation (eno2−) was earlier than that of wild type (WT) in Arabidopsis thaliana. Previous studies have shown that microRNAs (miRNAs) were vital in seed germination. After deep sequencing of small RNA, we found 590 differentially expressed miRNAs in total, of which 87 were significantly differentially expressed miRNAs. By predicting the target genes of miRNAs and analyzing the GO annotation, we have counted 18 genes related to seed germination, including ARF family, TIR1, INVC, RR19, TUDOR2, GA3OX2, PXMT1, and TGA1. MiR9736-z, miR5059-z, ath-miR167a-5p, ath-miR167b, ath-miR5665, ath-miR866-3p, miR10186-z, miR8165-z, ath-miR857, ath-miR399b, ath-miR399c-3p, miR399-y, miR163-z, ath-miR393a-5p, and ath-miR393b-5p are the key miRNAs regulating seed germination-related genes. Through KEGG enrichment analysis, we found that phytohormone signal transduction pathways were significantly enriched, and these miRNAs mentioned above also participate in the regulation of the genes in plant hormone signal transduction pathways, thus affecting the synthesis of plant hormones and further affecting the process of seed germination. This study laid the foundation for further exploration of the AtENO2 regulation for seed germination.
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