Promotion of the immunity of piglets to Hog cholera vaccine induced by shuffled pig interleukin-2 gene and CpG immunostimulatory sequences encapsulated in chitosan nanoparticles
Promotion of the immunity of piglets to Hog cholera vaccine induced by shuffled pig interleukin-2 gene and CpG immunostimulatory sequences encapsulated in chitosan nanoparticles
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壳聚糖纳米粒子封装猪白介素2基因和CpG免疫刺激序列改组诱导仔猪对猪霍乱疫苗的免疫力增强
DOI：
10.1016/j.provac.2010.03.010
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
R. Gao
中科院分区：
文献类型：
--
作者：
Xiao Yang;Wen;Wen;Jianlin Chen;X. Wan;Huan Zhang;Linwei Cai;Zezhou Wang;X. Lv;Hong;Liu Jiangling;R. Gao
将重组猪IL-2基因及其11个CpG基序亚克隆到真核表达质粒VR 1020和VR 1012中，分别命名为重组体VRIL-2S和VR 1C。将40头21日龄仔猪随机分为4组，分别肌肉注射0.5 mg VRIL 2S-CNP、VR 1C-CNP、VR 1020-CNP和CNP沿着注射猪瘟弱毒疫苗。免疫后每周采血检测免疫球蛋白、特异性抗体、白细胞介素、IFN-γ和免疫细胞的变化。结果发现，与对照组相比，VRIL 2S-CNP组和VR 1C-CNP组血清中免疫球蛋白、特异性抗体、IL-2、IL-6、IL-4和IFN-γ的含量显著升高（P<0.05），淋巴细胞和单核细胞数量也显著升高（P <0.05）。免疫后7、28 ~ 56 d，VRIL 2S-CNP组特异性抗体、IgM、伊加、IgG水平均显著高于VR 1C-CNP组（P<0.05）; VRIL 2S-CNP组IL-2、IFN-γ水平也显著高于VR 1C-CNP组（P<0.05）。VRIL 2S-CNP组淋巴细胞和单核细胞数量较VR 1C-CNP组有不同程度的增加（P<0.05）。试验期间，VRIL 2S-CNP处理组猪的平均增重也明显高于对照组。说明VRIL 2S-CNP和VR 1C-CNP对仔猪的体液免疫和细胞免疫均有显著的促进作用，且VRIL 2S比VR 1C-CNP能更好地诱导仔猪对猪瘟的免疫应答，说明CNP包埋重组猪IL-2基因是一种安全有效的猪瘟特异性免疫佐剂，有利于开发一种新的有效佐剂，提高猪对猪瘟的免疫力。
A novel shuffled pig IL-2 gene and immunostimulatory sequences containing 11 CpG motifs were subcloned into the eukaryotic expression plasmid VR1020 and VR1012, respectively designated as the recombinant VRIL2S and VR1C, then they were encapsulated in chitosan nanoparticles (CNP) prepared by ionotropic gelation method. Forty 21-day-old piglets were divided into four groups and intramuscularly injected respectively with 0.5mg VRIL2S-CNP, VR1C-CNP, VR1020-CNP and CNP along with the rabbit-attenuated swine fever virus vaccines. The bloods were weekly collected from the piglets after vaccination to detect the changes of immunoglobulins, specific antibodies, interleukins, IFN-γ and immune cells. The results were found that in comparison with the control VR1020-CNP and CNP group, the content of immunoglobulins, specific antibodies, IL-2, IL-6, IL-4 and IFN-γ significantly increased in the sera from the treated VRIL2S-CNP and VR1C-CNP group (P<0.05); the numbers of lymphocytes and monocytes also remarkably elevated in the treated groups (P<0.05). Meanwhile, the levels of specific antibody, IgM, IgA and IgG in the VRIL2S-CNP group were significantly higher than those of VR1C-CNP group respectively from 7 or 28 to 56 days after vaccination (P<0.05); so did the amount of IL-2 and IFN-γ in the VRIL2S-CNP group (P<0.05). Moreover, the number of lymphocytes and monocytes rose to different degrees in the VRIL2S-CNP treated pilglets compared to those of VR1C-CNP group (P<0.05). The average weight gain of in VRIL2S-CNP treated pigs was also obviously higher than that of the controls during the experimental period. These indicated that the humoral and cellular immunities of piglets were significantly promoted by VRIL2S-CNP and VR1C-CNP, and VRIL2S could provoked better immunities of piglets to Hog Cholera than VR1C-CNP, implying that the shuffled pig IL-2 gene entrapped with CNP is a safe and powerful adjuvant to boost the specific immunity of piglets against Hog cholera, facilitating the development of a novel effective adjuvant to improve the immunity of pig against Hog cholera.
DOI：
10.1073/pnas.82.24.8663
发表时间：
1985-12
影响因子：
11.1
作者：
G. H. Reem;N. Yeh;D. Urdal;P. Kilian;J. Farrar
通讯作者：
G. H. Reem;N. Yeh;D. Urdal;P. Kilian;J. Farrar
影响因子：
10.8
作者：
Mao, HQ;Roy, K;Leong, KW
通讯作者：
Leong, KW