Plasticity of lumbosacral propriospinal neurons is associated with the development of autonomic dysreflexia after thoracic spinal cord transection.
Plasticity of lumbosacral propriospinal neurons is associated with the development of autonomic dysreflexia after thoracic spinal cord transection.
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DOI：
10.1002/cne.21771
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发表时间：
2008-08-01
影响因子：
2.5
通讯作者：
Rabchevsky, Alexander G.
中科院分区：
文献类型：
--
作者：
Hou, Shaoping;Duale, Hanad;Cameron, Adrian A.;Abshire, Sarah M.;Lyttle, Travis S.;Rabchevsky, Alexander G.
关键词：
高于T6水平的完全胸（T）脊髓损伤（SCI）通常导致自主神经反射异常，这是一种通常由SCI水平以下的伤害性刺激触发的异常高血压状况。神经生长因子在腰骶脊髓的过度表达诱导了大量的伤害性盆腔内脏传入纤维的发芽，这与伤害性结直肠扩张引起的高血压增加有关。完全T4脊髓损伤后，我们评估了固有脊髓神经元的可塑性，将内脏输入吻向胸交感节前神经元。将顺行示踪剂生物素化葡聚糖胺（BDA）注射到损伤/非横断大鼠的腰骶背侧灰质连合（DGC）后立即（急性）或2周后（延迟）。在延迟或急性注射后1周或2周，分别在损伤脊髓的胸背侧灰质中发现比未横断脊髓的胸背侧灰质中更高密度（p<0.05）的BDA+纤维。为了证实，在损伤/非横断后2周将固蓝（FB）或霍乱毒素β亚单位（CTb）注射到T9背角中。运输1周后，在损伤的脊髓中相对于未横断的脊髓中定量了更多的逆行标记（p<0.05）的DGC固有脊髓神经元（T13-S1）。我们还监测了结直肠扩张后即刻早期基因c-fos的表达，发现损伤后整个DGC中c-Fos+细胞数量增加（p<0.01）。总的来说，这些结果意味着，与当地的初级传入纤维可塑性，损伤诱导的DGC神经元发芽可能是一个关键组成部分，在中继内脏感觉输入交感神经节前神经元，引起自主反射障碍后，高胸部SCI。
Complete thoracic (T) spinal cord injury (SCI) above the T6 level typically results in autonomic dysreflexia, an abnormal hypertensive condition commonly triggered by nociceptive stimuli below the level of SCI. Over-expression of nerve growth factor in the lumbosacral spinal cord induces profuse sprouting of nociceptive pelvic visceral afferent fibers that correlates with increased hypertension in response to noxious colorectal distension. After complete T4 SCI, we evaluated the plasticity of propriospinal neurons conveying visceral input rostrally to thoracic sympathetic preganglionic neurons. The anterograde tracer biotinylated dextran amine (BDA) was injected into the lumbosacral dorsal gray commissure (DGC) of injured/non-transected rats immediately after injury (acute) or 2 weeks later (delayed). At 1 or 2 weeks after delayed or acute injections, respectively, a higher density (p<0.05) of BDA+ fibers was found in thoracic dorsal gray matter of injured versus non-transected spinal cords. For corroboration, fast blue (FB) or cholera toxin subunit beta (CTb) was injected into the T9 dorsal horns 2 weeks post-injury/non-transection. After 1 week transport, more retrogradely-labeled (p<0.05) DGC propriospinal neurons (T13-S1) were quantified in injured versus non-transected cords. We also monitored immediate early gene, c-fos, expression following colorectal distension and found increased (p<0.01) c-Fos+ cell numbers throughout the DGC after injury. Collectively, these results imply that, in conjunction with local primary afferent fiber plasticity, injury-induced sprouting of DGC neurons may be a key constituent in relaying visceral sensory input to sympathetic preganglionic neurons that elicit autonomic dysreflexia after high thoracic SCI.
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影响因子：
5.3
作者：
Cameron, AA;Smith, GM;Rabchevsky, AG
通讯作者：
Rabchevsky, AG
影响因子：
3.3
作者：
Krenz, NR;Weaver, LC
通讯作者：
Weaver, LC
DOI：
10.1016/s0165-1838(98)00069-1
发表时间：
1998-07-15
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
Llewellyn-Smith, IJ;Arnolda, LF;Minson, JB
通讯作者：
Minson, JB
影响因子：
2.5
作者：
HANCOCK, MB;PEVETO, CA
通讯作者：
PEVETO, CA
影响因子：
4.4
作者：
Diaz-Ruiz, Araceli;Salgado-Ceballos, Hermelinda;Rios, Camilo
通讯作者：
Rios, Camilo