G1- and S-phase synthesis of histone H1 subtypes from mouse NIH fibroblasts and rat C6 glioma cells.
G1- and S-phase synthesis of histone H1 subtypes from mouse NIH fibroblasts and rat C6 glioma cells.
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来自小鼠 NIH 成纤维细胞和大鼠 C6 神经胶质瘤细胞的组蛋白 H1 亚型的 G1 期和 S 期合成。
DOI：
10.1021/bi00055a025
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
H. Lindner
中科院分区：
文献类型：
--
作者：
H. Talasz;W. Helliger;B. Puschendorf;H. Lindner
采用高效液相色谱技术和常规凝胶电泳相结合的方法，比较了同步化小鼠NIH 3 T3成纤维细胞和大鼠C6胶质瘤细胞在G 0、G1和S期组蛋白H1亚型的合成速率。在小鼠细胞系中，可检测到所有H1亚型，H1 a-H1 e，包括组蛋白H1（0）。而在大鼠细胞系中，未发现组蛋白H1 a，两种细胞系的H1 c和H1 e在静止状态下显示出与H1（0）相当的相对高的比活性。从G 0/G1阻断释放后，H1（0）的合成以及H1 c和H1 e的合成在短时间内增加。所有H1亚型在刺激后的同一时间具有其最大比活性。随着细胞从G 0/G1期进入S期，H1 a、H1b和H1 d的总H1比活性百分比增加，H1 c和H1 e的总H1比活性百分比保持相对恒定，而H1（0）的总H1比活性百分比降低。这些发现支持了我们的假设，即H1亚型可以分为三组，具有共同的代谢特征：一组由H1 a，H1b和H1 d组成;另一组由H1 c和H1 e组成;第三组由H1（0）组蛋白组成。此外，来自两个不同物种的相应H1亚型似乎在G1和S期具有相似的特异性活性。
The rates of synthesis of histone H1 subtypes in synchronized mouse NIH 3T3 fibroblasts were compared with those of rat C6 glioma cells during the G0, G1, and S phases by using a combination of HPLC techniques and conventional gel electrophoresis. In the mouse cell line, all H1 subtypes, H1a-H1e including histone H1(0), were detectable. In the rat cell line, however, no histone H1a was found. H1c and H1e from both cell lines show in the quiescent state a relatively high specific activity comparable with that of H1(0). After release from the G0/G1 block, the synthesis of H1(0) and likewise that of H1c and H1e increase for a short period. All H1 subtypes have their maximum specific activity at the same time after stimulation. The percentage of total H1 specific activity of H1a, H1b, and H1d increases, those of H1c and H1e remain relatively constant, and that of H1(0) decreases while cells cycle from the G0/G1 to the S phase. These findings support our assumption that H1 subtypes could be classified into three groups with common metabolic characteristics: one consists of H1a, H1b, and H1d; another of H1c and H1e; and a third of H1(0) histone. Moreover, the corresponding H1 subtypes from two different species seem to have similar specific activities during the G1 and S phases.
大鼠胸腺 H1 组蛋白高度磷酸化亚成分的表征。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Langan,TA
通讯作者：
Langan,TA
组蛋白 H1 亚组分和 H10 在非分裂细胞中的周转率不同。
影响因子：
2.9
作者：
Pehrson,JR;Cole,RD
通讯作者：
Cole,RD