Histone H1 subfractions and H10 turnover at different rates in nondividing cells.
Histone H1 subfractions and H10 turnover at different rates in nondividing cells.
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组蛋白 H1 亚组分和 H10 在非分裂细胞中的周转率不同。
DOI：
10.1021/bi00532a006
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Cole,RD
中科院分区：
文献类型：
--
作者：
Pehrson,JR;Cole,RD
材料与方法凝胶电泳。根据Laemmli（1970）的方法进行NaDodS 041-聚丙烯酰胺凝胶电泳;这些凝胶具有12.5%丙烯酰胺分离凝胶。二维凝胶使用乙酸-尿素-聚丙烯酰胺凝胶（Panyim和Chalkley，1969 a）作为第一维。这个凝胶被简单地染色以观察泳道。将轻度染色的凝胶在蒸馏水中浸泡3分钟，切出感兴趣的泳道。将这些条置于50 mL螺旋盖管中，并用30 mL 0.0625 M Tris和1% NaDodS 04（pH 6.8）平衡1 h。用新鲜的缓冲液再重复该过程1小时，并将平衡的条带浇铸到正常的NaDodS 〇 4堆积凝胶中。电泳照常进行，凝胶染色和脱色如前所述（Pehrson和科尔，1980）。在525 nm下扫描染色的凝胶。HI和HI 0的碱性磷酸酶处理。用冰冷的0.74 M PCA从整个神经母细胞瘤细胞中选择性提取HI和HI 0（Johns & Butler，1962）。将提取的组蛋白溶解在50 mM Tris（pH 8.2）中，并加入大肠杆菌碱性磷酸酶（Worthington，BAPF）至终浓度为1 mg/mL。将混合物在37 ℃温育16小时，通过加入3 × NaDodSO 4样品缓冲液（30%甘油、9%NaDodSO4和0.188MTris，pH6.8;为了溶解该混合物，必须将其加热至37 ℃）终止反应。蛋白质在NaDodS 04-聚丙烯酰胺凝胶中电泳。组蛋白的标记。如所述（Pehrson和科尔，1980），在100 × 20 mm组织培养皿上生长小鼠神经母细胞瘤细胞。快速分裂的细胞被标记，
Materials and MethodsGel Electrophoresis. NaDodS041-polyacrylamide gel electrophoresis was run according to the method of Laemmli (1970); these gels had a 12.5% acrylamide separating gel. Two-dimensional gels used an acetic acid-urea-polyacrylamide gel (Panyim & Chalkley, 1969a) for the first dimension. This gel was stained very briefly justto visualize the lanes. The lightly stained gel was soaked in distilled water for 3 min, and the lanes of interest were cut out. These strips were placed in 50-mL screw top tubes and equilibrated with 30 mL of 0.0625 M Tris and 1% NaDodS04, pH 6.8, for 1 h. This was repeated for another 1-h period with fresh buffer, and the equilibrated strip was cast into the normal NaDodS04 stacking gel. Electrophoresis was carried out as usual, and gels were stained and destained as previously described (Pehrson & Cole, 1980). Stained gels were scanned at 525 nm. Alkaline Phosphatase Treatment of HI and HI0. HI and HI0 were selectively extracted from whole neuroblastomacells with ice-cold 0.74 M PCA (Johns & Butler, 1962). The extracted histone was dissolved in 50 mM Tris, pH 8.2, and Escherichia coli alkaline phosphatase (Worthington, BAPF) was added to a final concentration of 1 mg/mL. The mixture was incubated at 37 C for 16 h, and the reaction was ter-minated by the addition of 3 X NaDodS04 sample buffer (30% glycerol, 9% NaDodS04, and 0.188 M Tris, pH 6.8; to dissolve this mixture, it must be warmed to 37 C). The proteins were electrophoresed in NaDodS04-polyacrylamide gels. Labeling of Histones. Mouse neuroblastoma cells were grown as described (Pehrson & Cole, 1980), on 100 X 20 mm tissue culture dishes. Rapidly dividing cells were labeled when
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影响因子：
2.9
作者：
R. Balhorn;W. O. Rieke;R. Chalkley
通讯作者：
R. Chalkley
组蛋白 H1 从整个 HeLa 核中的选择性置换：微球菌核酸酶探测对原位染色质结构的影响。
影响因子：
2.9
作者：
G. Lawson;R. Cole
通讯作者：
R. Cole
富含赖氨酸的组蛋白氨基末端区域的氨基酸序列和序列变异性。
DOI：
10.1016/s0021-9258(19)45870-5
发表时间：
1971
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. C. Rall;R. Cole
通讯作者：
R. Cole
DOI：
10.1016/0045-6039(74)90035-9
发表时间：
1974-01-01
期刊：
CELL DIFFERENTIATION
影响因子：
--
作者：
APPELS, R;RINGERTZ, NR
通讯作者：
RINGERTZ, NR
DOI：
10.1073/pnas.57.2.409
发表时间：
1967-01-01
影响因子：
11.1
作者：
ROBBINS, E;BORUN, TW
通讯作者：
BORUN, TW