Myristoylation of human immunodeficiency virus type 1 gag protein is required for efficient env protein transportation to the surface of cells.
Myristoylation of human immunodeficiency virus type 1 gag protein is required for efficient env protein transportation to the surface of cells.
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人类免疫缺陷病毒 1 型 gag 蛋白的肉豆蔻酰化是 env 蛋白有效转运至细胞表面所必需的。
DOI：
10.1006/bbrc.2001.4696
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发表时间：
2001
影响因子：
3.1
通讯作者：
S. Shoji
中科院分区：
文献类型：
--
作者：
T. Shiraishi;S. Misumi;M. Takama;I. Takahashi;S. Shoji
人类免疫缺陷病毒1型（HIV-1） Pr55(gag) n端区域高度保守的氨基酸被认为对肉豆蔻酸的附着至关重要。我们之前报道过，通过n -肉豆蔻酰甘氨酸二乙基缩醛阻断Pr55（gag）的n -肉豆蔻酰化，在细胞表面检测不到env蛋白。在这里，我们构建了一个突变体，用丙氨酸取代Pr55（gag）的n端甘氨酸，以证明Pr55（gag）的n肉豆蔻酰化是有效的环境蛋白运输到细胞表面所必需的。电镜观察发现，转染肉豆蔻烯甲基化缺陷表型的Jurkat细胞表面env蛋白的表达水平显著降低。此外，转染肉豆蔻酰基化缺陷表型的Jurkat细胞失去了包膜介导的细胞间融合能力。结果表明，HIV-1 gag蛋白的n -肉豆蔻酰基化对于有效地将环境蛋白转运到细胞表面是必要的。
Highly conserved amino acids in the N-terminal region of the human immunodeficiency virus type 1 (HIV-1) Pr55(gag) are recognized to be critical for the attachment of myristic acid. We previously reported that the env protein was not detected on the cell surface by blocking of N-myristoylation of Pr55(gag) with N-myristoyl glycinal diethylacetal. Here, we constructed a mutant by substituting the N-terminal glycine of Pr55(gag) with alanine to demonstrate that N-myristoylation of Pr55(gag) is required for efficient env protein transportation to the cell surface. The expression level of the env protein on the surface of Jurkat cells transfected with the myristoylation-defective phenotype was observed to be significantly reduced by electron microscopic analyses with a gold-labeled monoclonal antibody against the env protein. In addition, Jurkat cells transfected with the myristoylation-defective phenotype lost the ability of envelope-mediated cell-to-cell fusion. The results suggest that N-myristoylation of the HIV-1 gag protein is necessary for efficient env protein transportation to the cell surface.
DOI：
10.1073/pnas.86.15.5781
发表时间：
1989-08-01
影响因子：
11.1
作者：
GOTTLINGER, HG;SODROSKI, JG;HASELTINE, WA
通讯作者：
HASELTINE, WA
DOI：
10.1073/pnas.87.2.523
发表时间：
1990-01-01
影响因子：
11.1
作者：
BRYANT, M;RATNER, L
通讯作者：
RATNER, L