Receptor-Like Kinase LYK9 in Pisum sativum L. Is the CERK1-Like Receptor that Controls Both Plant Immunity and AM Symbiosis Development.
Receptor-Like Kinase LYK9 in Pisum sativum L. Is the CERK1-Like Receptor that Controls Both Plant Immunity and AM Symbiosis Development.
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DOI：
10.3390/ijms19010008
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发表时间：
2017-12-21
影响因子：
5.6
通讯作者：
Dolgikh EA
中科院分区：
文献类型：
--
作者：
Leppyanen IV;Shakhnazarova VY;Shtark OY;Vishnevskaya NA;Tikhonovich IA;Dolgikh EA
植物能够区分和响应来自病原真菌和共生真菌的结构相关的壳寡糖（CO）信号。在模式植物拟南芥（Arabidopsis thaliana）和水稻（Oryza sativa）中，LysM受体样激酶（LysM-RLK）AtCERK 1和OsCERK 1（几丁质激发子受体激酶1）被证明参与对CO信号的响应。系统发育分析表明，豌豆Pisum sativum L. LysM-RLK PsLYK 9被选为可能的候选者，因为它在CERK 1样受体上发挥作用。通过RNA干扰对PsLyk 9基因的敲低调控导致对真菌病原体黄色镰刀菌的易感性增加。PsPAL 2、PsPR 10防御反应基因在PsLyk 9 RNAi根中的转录水平显著降低。PsLYK 9参与识别短链CO作为丛枝菌根（AM）真菌的大多数信号，也进行了评估。在短链CO 5处理的PsLyk 9基因敲减的转基因根中，观察到AM共生标记基因（PsDELLA 3、PsNSP 2、PsDWARF 27）的下调。这些结果清楚地表明PsLYK 9似乎参与豌豆根中CO的感知和随后的信号转导。这使我们能够得出结论，PsLYK 9是豌豆中最有可能参与植物免疫和AM共生形成控制的CERK 1样受体。
Plants are able to discriminate and respond to structurally related chitooligosaccharide (CO) signals from pathogenic and symbiotic fungi. In model plants Arabidopsis thaliana and Oryza sativa LysM-receptor like kinases (LysM-RLK) AtCERK1 and OsCERK1 (chitin elicitor receptor kinase 1) were shown to be involved in response to CO signals. Based on phylogenetic analysis, the pea Pisum sativum L. LysM-RLK PsLYK9 was chosen as a possible candidate given its role on the CERK1-like receptor. The knockdown regulation of the PsLyk9 gene by RNA interference led to increased susceptibility to fungal pathogen Fusarium culmorum. Transcript levels of PsPAL2, PsPR10 defense-response genes were significantly reduced in PsLyk9 RNAi roots. PsLYK9’s involvement in recognizing short-chain COs as most numerous signals of arbuscular mycorrhizal (AM) fungi, was also evaluated. In transgenic roots with PsLyk9 knockdown treated with short-chain CO5, downregulation of AM symbiosis marker genes (PsDELLA3, PsNSP2, PsDWARF27) was observed. These results clearly indicate that PsLYK9 appears to be involved in the perception of COs and subsequent signal transduction in pea roots. It allows us to conclude that PsLYK9 is the most likely CERK1-like receptor in pea to be involved in the control of plant immunity and AM symbiosis formation.
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影响因子：
6.9
作者：
Azarakhsh M;Kirienko AN;Zhukov VA;Lebedeva MA;Dolgikh EA;Lutova LA
通讯作者：
Lutova LA
影响因子：
9.4
作者：
Delaux, Pierre-Marc;Becard, Guillaume;Combier, Jean-Philippe
通讯作者：
Combier, Jean-Philippe
影响因子：
7.4
作者：
Arrighi, Jean-Francois;Barre, Annick;Gough, Clare
通讯作者：
Gough, Clare
影响因子：
11.6
作者：
Gonzalez-Rizzo, Silvina;Crespi, Martin;Frugier, Florian
通讯作者：
Frugier, Florian
影响因子：
10.7
作者：
Hall, Barry G.
通讯作者：
Hall, Barry G.