Synthesis and Characterization of an Injectable and Hydrophilous Expandable Bone Cement Based on Poly(methyl methacrylate).
Synthesis and Characterization of an Injectable and Hydrophilous Expandable Bone Cement Based on Poly(methyl methacrylate).
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基于聚甲基丙烯酸甲酯的可注射亲水可膨胀骨水泥的合成和表征。
DOI：
10.1021/acsami.7b12983
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Lei Wei
中科院分区：
文献类型：
--
作者：
Yang Zhao;Chen Lei;Hao Yuxin;Zang Yuan;Zhao Xiong;Shi Lei;Zhang Yang;Feng Yafei;Xu Chao;Wang Faqi;Wang Xinli;Wang Bowen;Liu Chenxin;Tang Yufei;Wu Zixiang;Lei Wei
聚（甲基丙烯酸甲酯）（PMMA）是最常见的骨水泥，已在骨科手术（如椎体成形术）中用作移植物替代物。然而，聚合过程中的收缩和传统PMMA骨水泥的高弹性模量引起的骨-骨水泥界面中的不良小裂纹显著增加了术后椎体再骨折的风险。因此，在此，基于PMMA商业水泥（Mendec Spine Resin）、丙烯酸（AA）和苯乙烯（St）合成了可膨胀骨水泥。合成的两种骨水泥（PMMA-PAA，PMMA-PAA-PSt）在体外表现出良好的体积膨胀性，在兔股骨腔缺损中表现出良好的骨水泥接触力。新型骨水泥的弹性模量和抗压强度均低于PMMA。此外，体外分析表明，新的骨水泥比PMMA具有更低的细胞毒性，包括Sprague-Dawly大鼠源性成骨细胞的上级增殖和更低的凋亡率。蛋白质表达和RT-PCR分析的成骨特异性基因的蛋白质印迹进行了SD大鼠来源的成骨细胞从PMMA和新的水泥膜;结果表明，新的水泥增强了成骨特异性基因的表达。扫描电子显微镜显示，与PMMA盘相比，新骨水泥盘上成骨细胞的形态和附着得到改善。此外，来自兔内侧股骨髁腔缺损模型的骨-骨水泥界面的组织学形态显示，与PMMA骨水泥组的轻微裂纹相比，新骨水泥组与骨直接且内聚接触。12周后，在新骨水泥中发现了新骨生长到骨水泥中的迹象，从而表明体内成骨能力。因此，基于PMMA和聚丙烯酸（PAA）的合成可膨胀骨水泥是椎体成形术的有希望的候选者。
Poly(methyl methacrylate) (PMMA), the most common bone cement, has been used as a graft substitute in orthopedic surgeries such as vertebroplasty. However, an undesirable minor crack in the bone-cement interface provoked by shrinkage during polymerization and high elastic modulus of conventional PMMA bone cement dramatically increases the risk of vertebral body refracture postsurgery. Thus, herein, a hydrophilous expandable bone cement was synthesized based on a PMMA commercial cement (Mendec Spine Resin), acrylic acid (AA), and styrene (St). The two synthesized cements (PMMA-PAA, PMMA-PAA-PSt) showed excellent volumetric swelling in vitro and cohesive bone-cement contact in rabbit femur cavity defect. The elastic modulus and compressive strength of the new cements were lower than PMMA. Furthermore, the in vitro analysis indicated that the new cements had lower cytotoxicity than PMMA, including superior proliferation and lower apoptotic rates of Sprague-Dawly rat-derived osteoblasts. Western blotting for protein expression and RT-PCR analysis of osteogenesis-specific genes were conducted on SD rat-derived osteoblasts from both PMMA and new cements films; the results showed that new cements enhanced the expression of osteogenesis-specific genes. Scanning electron microscopy demonstrated improved morphology and attachment of osteoblast on new cement discs compared to the PMMA discs. Additionally, the histological morphologies of the bone-cement interface from the rabbit medial femoral condyle cavity defect model revealed direct and cohesive contact with the bone in the new cement groups in contrast to a minor crack in the PMMA cement group. The sign of a new bone growing into the cement has been found in the new cements after 12 weeks, thereby indicating the osteogenic capacity in vivo. In conclusion, the synthesized hydrophilous expandable bone cements based on PMMA and poly(acrylic acid) (PAA) are promising candidates for vertebroplasty.
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影响因子：
3
作者：
Y. Nien;Jason E Chen
通讯作者：
Y. Nien;Jason E Chen
DOI：
10.12659/msm.893845
发表时间：
2015-04-23
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Yang J;Zhang K;Zhang S;Fan J;Guo X;Dong W;Wang S;Chen Y;Yu B
通讯作者：
Yu B
影响因子：
16.6
作者：
Uemura T;Kaseda T;Sasaki Y;Inukai M;Toriyama T;Takahara A;Jinnai H;Kitagawa S
通讯作者：
Kitagawa S
DOI：
10.1002/1097-4636(200010)52:1
发表时间：
2000-10
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
C. Tsai;R. Huang;H. Sung;H. Liang
通讯作者：
C. Tsai;R. Huang;H. Sung;H. Liang
影响因子：
11.4
作者：
Mohan, Arya J.;Sekhar, Vini C.;Nampoothiri, K. Madhavan
通讯作者：
Nampoothiri, K. Madhavan