Organs-on-chips with integrated electrodes for trans-epithelial electrical resistance (TEER) measurements of human epithelial barrier function.
Organs-on-chips with integrated electrodes for trans-epithelial electrical resistance (TEER) measurements of human epithelial barrier function.
复制标题
具有集成电极的器官芯片，用于测量人体上皮屏障功能的跨上皮电阻 (TEER)。
DOI：
10.1039/c7lc00155j
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发表时间：
2017-06-27
期刊：
影响因子：
6.1
通讯作者：
Ingber DE
中科院分区：
文献类型：
--
作者：
Henry OYF;Villenave R;Cronce MJ;Leineweber WD;Benz MA;Ingber DE
跨上皮电阻（TEER）被广泛用作用于测量在体外静态条件下培养的上皮单层的完整性的实验读数和质量控制测定，然而，没有标准方法用于其应用于微流体器官芯片（器官芯片）培养。在这里，我们描述了一种新的微流控器官芯片设计，包含嵌入式电极，我们证明了其效用评估的屏障功能的形成和破坏内的人类肺气道芯片内衬由完全分化的粘膜纤毛人类气道上皮细胞和人类肠道芯片内衬肠上皮细胞。这些集成电极的芯片能够实时、无创地监测TEER，并可用于测量几乎任何类型培养细胞的屏障功能。人肺气道上皮细胞长期成熟过程中的跨上皮电阻（TEER）监测和器官芯片中的实验操作
Trans-Epithelial Electrical Resistance (TEER) is broadly used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured under static conditions in vitro, however, there is no standard methodology for its application to microfluidic Organ-on-a-Chip (Organ Chip) cultures. Here, we describe a new microfluidic Organ Chip design that contains embedded electrodes, and we demonstrate its utility for assessing formation and disruption of barrier function both within a human Lung Airway Chip lined by a fully differentiated mucociliary human airway epithelium and in a human Gut Chip lined by intestinal epithelial cells. These chips with integrated electrodes enable real-time, non-invasive monitoring of TEER and can be applied to measure barrier function in virtually any type of cultured cell. Trans-epithelial electrical resistance (TEER) monitoring of human lung airway epithelium during long term maturation and experimental manipulation in an Organ-on-a-Chip
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影响因子：
3.8
作者：
Wang YI;Abaci HE;Shuler ML
通讯作者：
Shuler ML
影响因子：
7.4
作者：
Douville, Nicholas J.;Tung, Yi-Chung;Li, Ran;Wang, Jack D.;El-Sayed, Mohamed E. H.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
19
作者：
Huh, Dongeun;Hamilton, Geraldine A.;Ingber, Donald E.
通讯作者：
Ingber, Donald E.
DOI：
10.1177/2211068214561025
发表时间：
2015-04
期刊：
Journal of laboratory automation
影响因子：
--
作者：
Srinivasan B;Kolli AR;Esch MB;Abaci HE;Shuler ML;Hickman JJ
通讯作者：
Hickman JJ
影响因子：
3.7
作者：
Wegener, J;Keese, CR;Giaever, I
通讯作者：
Giaever, I