Characterization of a bifunctional O- and N-glucosyltransferase from Vitis vinifera in glucosylating phenolic compounds and 3,4-dichloroaniline in Pichia pastoris and Arabidopsis thaliana.
Characterization of a bifunctional O- and N-glucosyltransferase from Vitis vinifera in glucosylating phenolic compounds and 3,4-dichloroaniline in Pichia pastoris and Arabidopsis thaliana.
复制标题
DOI：
10.1371/journal.pone.0080449
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yao QH
中科院分区：
文献类型：
--
作者：
Xu ZS;Xue W;Xiong AS;Lin YQ;Xu J;Zhu B;Zhao W;Peng RH;Yao QH
2,4,5-Trichlorophenol, 2,6-dimethylphenol, 3-methylcatechol, phenol, hydroquinone, catechol, and 3,4-dichloroaniline are present in the environment and are risky to humans and animals because of their wide applications in many industries. In this study, a putative uridine diphosphate glucose-dependent glycosyltransferase from Vitis vinifera (VvUGT72B1) displayed high O-glucosyltransferase or N-glucosyltransferase activity toward all these xenbiotics and was able to enhance the resistance of P. pastoris to them. Compared with wild-type Arabidopsis plants, VvUGT72B1-transgenic Arabidopsis plants showed higher resistance to all the xenobiotics except for phenol and exhibited higher removal efficiencies against all xenobiotics. Glucosides of 3-methylcatechol, 2,6-dimethylphenol, phenol, and 3,4-dichloroaniline were exported to the surrounding media by Arabidopsis plants while transgenic Arabidopsis plants exported more glucosides than wild-type Arabidopsis plants. Our findings have the potential to provide a broader spectrum remediation strategy for the phytoremoval and degradation of phenolic compounds and 3,4-dichloroaniline than previous works.
登录
查看更多内容
影响因子：
7.4
作者：
Liao, Yang;Zhou, Xiao;Kuai, Benke
通讯作者：
Kuai, Benke
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
10.4
作者：
Dietz, AC;Schnoor, JL
通讯作者：
Schnoor, JL
影响因子：
7.2
作者：
Brazier-Hicks, M;Edwards, R
通讯作者：
Edwards, R
影响因子：
1.5
作者：
Hard, GC;Whysner, J;Williams, GM
通讯作者：
Williams, GM