Pseudomonas aeruginosa adaptation to lungs of cystic fibrosis patients leads to lowered resistance to phage and protist enemies.
Pseudomonas aeruginosa adaptation to lungs of cystic fibrosis patients leads to lowered resistance to phage and protist enemies.
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DOI：
10.1371/journal.pone.0075380
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Buckling A
中科院分区：
文献类型：
--
作者：
Friman VP;Ghoul M;Molin S;Johansen HK;Buckling A
致病的生活方式可能导致与寄主物种高度专业化的相互作用，潜在地导致在其他生态环境中的适应性权衡。在这里，我们研究了环境传播的细菌病原体铜绿假单胞菌对囊性纤维化(CF)患者的适应如何影响其在天然噬菌体(14/1，ΦKZ，PNM和Pt7)和原生生物(嗜热四膜虫和多食棘阿米巴)存在下的生存。我们发现，从相对较近的间歇性定植患者(1-25个月)分离的大多数细菌，具有天生的抗噬菌体能力，对原生生物具有高度毒性。相反，从长期慢性感染患者(2-23年)中分离出的细菌，在抵抗噬菌体和杀死原生生物方面效率较低。此外，慢性分离物对蜡蛾幼虫的杀灭作用降低，可能是由于其较弱的体外生长和蛋白酶表达所致。这些结果表明，铜绿假单胞菌对CF型肺的长期适应可能会与其在存在微生物敌人的水环境水库中的生存进行权衡，而较低的毒力可能会减少病原体感染昆虫媒介的机会；这两个因素都可能导致较差的环境传播。从应用的角度来看，噬菌体疗法可能对通常以多重耐药为特征的慢性铜绿假单胞菌肺部感染有用：慢性分离株对噬菌体的抵抗力最低，它们的不良生长可能会减缓有益耐药突变的出现。
Pathogenic life styles can lead to highly specialized interactions with host species, potentially resulting in fitness trade-offs in other ecological contexts. Here we studied how adaptation of the environmentally transmitted bacterial pathogen, Pseudomonas aeruginosa, to cystic fibrosis (CF) patients affects its survival in the presence of natural phage (14/1, ΦKZ, PNM and PT7) and protist (Tetrahymena thermophila and Acanthamoebae polyphaga) enemies. We found that most of the bacteria isolated from relatively recently intermittently colonised patients (1–25 months), were innately phage-resistant and highly toxic for protists. In contrast, bacteria isolated from long time chronically infected patients (2–23 years), were less efficient in both resisting phages and killing protists. Moreover, chronic isolates showed reduced killing of wax moth larvae (Galleria mellonella) probably due to weaker in vitro growth and protease expression. These results suggest that P. aeruginosa long-term adaptation to CF-lungs could trade off with its survival in aquatic environmental reservoirs in the presence of microbial enemies, while lowered virulence could reduce pathogen opportunities to infect insect vectors; factors that are both likely to result in poorer environmental transmission. From an applied perspective, phage therapy could be useful against chronic P. aeruginosa lung infections that are often characterized by multidrug resistance: chronic isolates were least resistant to phages and their poor growth will likely slow down the emergence of beneficial resistance mutations.
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影响因子：
3.1
作者：
Johansen, HK;Norregaard, L;Hoiby, N
通讯作者：
Hoiby, N
影响因子：
4.4
作者：
Hosseinidoust, Zeinab;Tufenkji, Nathalie;van de Ven, Theo G. M.
通讯作者：
van de Ven, Theo G. M.
影响因子：
3.7
作者：
Bjarnsholt T;Jensen PØ;Jakobsen TH;Phipps R;Nielsen AK;Rybtke MT;Tolker-Nielsen T;Givskov M;Høiby N;Ciofu O;Scandinavian Cystic Fibrosis Study Consortium
通讯作者：
Scandinavian Cystic Fibrosis Study Consortium
影响因子：
6.4
作者：
Alemayehu D;Casey PG;McAuliffe O;Guinane CM;Martin JG;Shanahan F;Coffey A;Ross RP;Hill C
通讯作者：
Hill C
影响因子：
6.4
作者：
Debarbieux, Laurent;Leduc, Dominique;Touqui, Lhousseine
通讯作者：
Touqui, Lhousseine