Early events in DNA replication require cyclin E and are blocked by p21CIP1.
Early events in DNA replication require cyclin E and are blocked by p21CIP1.
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DOI：
10.1083/jcb.130.4.755
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发表时间：
1995-08
影响因子：
7.8
通讯作者：
KIRSCHNER, MW
中科院分区：
文献类型：
--
作者：
JACKSON, PK;CHEVALIER, S;PHILIPPE, M;KIRSCHNER, MW
利用细胞周期蛋白E和抑制蛋白p21WAF/CIP1的免疫耗竭，我们证明了细胞周期蛋白E与CDK2结合是单链底物上复制的延伸期所必需的。虽然细胞周期蛋白E/CDK2可能是p21抑制精子DNA复制的主要靶点，但p21可以通过依赖于增殖细胞核抗原的机制抑制单链复制。虽然Cyclin E/CDK2复合体似乎在DNA复制的启动中起作用，但另一种CDK激酶，可能是Cyclin A/CDK，可能参与了控制从起始到延伸的切换的后续步骤。大量母体细胞周期蛋白E蛋白的提供表明复制调控因子的存在，这解释了在早期胚胎细胞周期中缺乏复制所需的蛋白质合成。
Using immunodepletion of cyclin E and the inhibitor protein p21WAF/CIP1, we demonstrate that the cyclin E protein, in association with Cdk2, is required for the elongation phase of replication on single-stranded substrates. Although cyclin E/Cdk2 is likely to be the major target by which p21 inhibits the initiation of sperm DNA replication, p21 can inhibit single-stranded replication through a mechanism dependent on PCNA. While the cyclin E/Cdk2 complex appears to have a role in the initiation of DNA replication, another Cdk kinase, possibly cyclin A/Cdk, may be involved in a later step controlling the switch from initiation to elongation. The provision of a large maternal pool of cyclin E protein shows that regulators of replication are constitutively present, which explains the lack of a protein synthesis requirement for replication in the early embryonic cell cycle.
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影响因子：
64.5
作者：
ELDEIRY, WS;TOKINO, T;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
7.8
作者：
KOBAYASHI, H;MINSHULL, J;HUNT, T
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HUNT, T
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56.9
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DULIC, V;LEES, E;REED, SI
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REED, SI
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HINDS, PW;MITTNACHT, S;WEINBERG, RA
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WEINBERG, RA
影响因子：
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作者：
GU, Y;TURCK, CW;MORGAN, DO
通讯作者：
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