Differential transcription profiles in Aedes aegypti detoxification genes after temephos selection.
Differential transcription profiles in Aedes aegypti detoxification genes after temephos selection.
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DOI：
10.1111/imb.12073
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发表时间：
2014-04
影响因子：
2.6
通讯作者：
Black WC 4th
中科院分区：
文献类型：
--
作者：
Saavedra-Rodriguez K;Strode C;Flores AE;Garcia-Luna S;Reyes-Solis G;Ranson H;Hemingway J;Black WC 4th
The mosquito Aedes aegypti is the main vector of Dengue and Yellow Fever flaviviruses. The organophosphate insecticide temephos is a larvicide that is used globally to control Ae. aegypti populations; many of which have in turn evolved resistance. Target site alteration in the acetylcholine esterase of this species has not being identified. Instead, we tracked changes in transcription of metabolic detoxification genes using the Ae. aegypti ‘Detox Chip’ microarray during five generations of temephos selection. We selected for temephos resistance in three replicates in each of six collections, five from México, and one from Perú. The response to selection was tracked in terms of lethal concentrations (LC50). Uniform upregulation was seen in the epsilon class glutathione-S-transferase genes (eGSTs) in strains from México prior to laboratory selection, while eGSTs in the Iquitos Perú strain became upregulated following five generations of temephos selection. While expression of many esterase genes (CCE) increased with selection, no single esterase was consistently upregulated and this same pattern was noted in the cytochrome P450 genes (CYP) and in other genes involved in reduction or oxidation of xenobiotics. Bioassays using GST, CCE and CYP inhibitors suggest that various CCE instead of GSTs are the main metabolic mechanism conferring resistance to temephos. We show that temephos selected strains show no cross resistance to permethrin and that genes associated with temephos selection are largely independent of those selected with permethrin in a previous study.
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DOI：
10.1590/s0074-02762003000500020
发表时间：
2003-07-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Macoris, Maria de Lourdes G;Andrighetti, Maria Teresa M;Bracco, José Eduardo
通讯作者：
Bracco, José Eduardo
影响因子：
1
作者：
Lozano-Fuentes, Saul;Saavedra-Rodriguez, Karla;Eisen, Lars
通讯作者：
Eisen, Lars
DOI：
10.1590/s0074-02762010000100019
发表时间：
2010-02-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Llinás, G Albrieu;Seccacini, E;Licastro, S
通讯作者：
Licastro, S
影响因子：
3.8
作者：
Huang, HS;Hu, NT;Sun, CN
通讯作者：
Sun, CN
影响因子：
3.8
作者：
Lumjuan, Nongkran;Rajatileka, Shavanthi;Ranson, Hilary
通讯作者：
Ranson, Hilary