Elucidation of Lignin and Polysaccharide Linkages in Wheat Straw by 2H/13C Isotopic Tracer
Elucidation of Lignin and Polysaccharide Linkages in Wheat Straw by 2H/13C Isotopic Tracer
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通过 2H/13C 同位素示踪剂阐明麦秆中的木质素和多糖连接
DOI：
10.15376/biores.18.1.550-569
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发表时间：
2022-11
期刊：
影响因子：
1.5
通讯作者：
Gongxia Zhang
中科院分区：
文献类型：
--
作者：
Yimin Xie;Xudong Chen;Kai Zhang;Sheng Cui;Gongxia Zhang
To elucidate chemical linkages between lignin and polysaccharides, theaqueous mixed solutions of coniferin-[a- 13C], syringin-[a-13C],D-glucose-[6-2H], and phenylalanine ammonia-lyase inhibitor were injected into aliving wheat stalk. Internode tissues with high abundance of 2H-13C werecollected.The milled wood lignin, lignin-carbohydrate complex (LCC), andresidual LCC(R-LCC) with enrichment of 2H-13C were isolated. The 13cand 2H abundances showed that the lignin and polysaccharides ofinternode tissues were labeled by 13C and 2H, respectively. Analysis withcarbon-13 nuclear magnetic resonance (13C-NMR)showed that ketal andbenzyl ether bonds were formed between a-C of lignin and carbohydrates.The R-LCC and LCC were further treated with enzymes to obtainenzymatic degraded R-LCC(ED-R-LCC) and enzymatic degraded LCc(ED-LCC). 13C-NMR spectra of ED-LCC showed that the a-C of lignin sidechain was combined with 6-C of carbohydrates by ether, ester, and ketallinkages.1H-NMR differential spectra of ED-LCCs revealed an LC linkageof benzyl ether bond.Glucan-lignin(En-R-GL) and xylan-lignin(En-R-XL)complexes were separated from ED-R-LCC by ionic liquid.A part of lignina-C was linked to cellulose 6-C by benzyl ether and a-ketal linkages. 13C-NMR spectra of En-R-XL showed there were a-benzyl ether and a-ketalbonds between lignin and xylan..
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DOI：
--
发表时间：
2001
期刊：
--
影响因子：
--
作者：
Olov Karlsson;B. Pettersson;U. Westermark
通讯作者：
Olov Karlsson;B. Pettersson;U. Westermark
DOI：
10.1111/tpj.12124
发表时间：
2013-04
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Du X;Gellerstedt G;Li J
通讯作者：
Li J
影响因子：
--
作者：
BJORKMAN, A
通讯作者：
BJORKMAN, A
影响因子：
4.3
作者：
Balakshin, Mikhail;Capanema, Ewellyn;Jameel, Hasan
通讯作者：
Jameel, Hasan
DOI：
10.1515/hf.2007.097
发表时间：
2007-11
期刊：
--
影响因子：
--
作者：
G. Henriksson;M. Lawoko;M. E. E. Martín-M.-E.-E.-Martín-2110742865;G. Gellerstedt
通讯作者：
G. Henriksson;M. Lawoko;M. E. E. Martín-M.-E.-E.-Martín-2110742865;G. Gellerstedt