Universal fractionation of lignin-carbohydrate complexes (LCCs) from lignocellulosic biomass: an example using spruce wood.
Universal fractionation of lignin-carbohydrate complexes (LCCs) from lignocellulosic biomass: an example using spruce wood.
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DOI：
10.1111/tpj.12124
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发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Du X;Gellerstedt G;Li J
开发一种普遍适用的方法对来自所有类型的木质纤维素生物质的木质素-碳水化合物复合物（LCC）进行分级和灵敏的木质素表征，无论是天然的还是经过各种类型的加工，都具有理论和实践意义。在本研究中，以前报道的分馏方法，适用于桉树（硬木）和亚麻（非木材）进一步改进，通过引入一个额外的步骤氢氧化钡沉淀分离富含甘露聚糖的LCC（葡甘露聚糖-木质素，GML），以适应软木物种以及。云杉木材用作软木样品。如回收率和组成分析所示，所有的木质素在三个LCC级分中回收：富含葡聚糖的级分（葡聚糖-木质素，GL）、富含甘露聚糖的级分（GML）和富含木聚糖的级分（木聚糖-木质素，XL）。所有LCC均具有高分子量，并且在二氧六环/水溶液中不溶或几乎不溶。碳水化合物和木质素信号在1H NMR，13 C CP-MAS NMR和正常或高灵敏度2D HSQC NMR分析中观察到。因此，每个LCC级分中的碳水化合物和木质素组分被认为是化学键合的，而不是彼此物理混合的。三个LCC馏分被发现是明显不同的，从彼此在其木质素结构方面，揭示了高灵敏度的分析，thiocidolysis-GC，thiocidolysis-SEC和热解-GC。
It is of both theoretical and practical importance to develop a universally applicable approach for the fractionation and sensitive lignin characterization of lignin–carbohydrate complexes (LCCs) from all types of lignocellulosic biomass, both natively and after various types of processing. In the present study, a previously reported fractionation approach that is applicable for eucalyptus (hardwood) and flax (non-wood) was further improved by introducing an additional step of barium hydroxide precipitation to isolate the mannan-enriched LCC (glucomannan-lignin, GML), in order to suit softwood species as well. Spruce wood was used as the softwood sample. As indicated by the recovery yield and composition analysis, all of the lignin was recovered in three LCC fractions: a glucan-enriched fraction (glucan-lignin, GL), a mannan-enriched fraction (GML) and a xylan-enriched fraction (xylan-lignin, XL). All of the LCCs had high molecular masses and were insoluble or barely soluble in a dioxane/water solution. Carbohydrate and lignin signals were observed in 1H NMR, 13C CP-MAS NMR and normal- or high-sensitivity 2D HSQC NMR analyses. The carbohydrate and lignin constituents in each LCC fraction are therefore believed to be chemically bonded rather than physically mixed with one another. The three LCC fractions were found to be distinctly different from each other in terms of their lignin structures, as revealed by highly sensitive analyses by thioacidolysis-GC, thioacidolysis-SEC and pyrolysis-GC.
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影响因子：
4.3
作者：
Balakshin, Mikhail;Capanema, Ewellyn;Jameel, Hasan
通讯作者：
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影响因子：
2.4
作者：
CHANG, HM;COWLING, EB;MIKSCHE, G
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MIKSCHE, G
影响因子：
2.9
作者：
Kawamoto, Haruo;Horigoshi, Sunao;Saka, Shiro
通讯作者：
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影响因子：
7.2
作者：
Lu, FC;Ralph, J
通讯作者：
Ralph, J
影响因子：
2.4
作者：
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通讯作者：
WANNSTROM, S