Peripheral Blood Lymphocytes as Target Cells of Retroviral Vector-Mediated Gene Transfer
Peripheral Blood Lymphocytes as Target Cells of Retroviral Vector-Mediated Gene Transfer
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外周血淋巴细胞作为逆转录病毒载体介导的基因转移的靶细胞
DOI：
10.1182/blood.v83.7.1988.bloodjournal8371988
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发表时间：
1994
期刊：
影响因子：
20.3
通讯作者：
C. Bordignon
中科院分区：
文献类型：
--
作者：
F. Mavilio;G. Ferrari;S. Rossini;N. Nobili;C. Bonini;G. Casorati;C. Traversari;C. Bordignon
用这四种载体转染人骨髓和淋巴造血细胞系，分析基因转移效率、载体原病毒的整合和稳定性，以及lnfr在RNA和蛋白水平上的表达。在转导的ETROVIRAL载体上，荧光激活细胞分选分析lnfr和细胞系特异性细胞表面标记的共表达是一种有效且相对安全的将外源DNA转移到体细胞的工具。逆转录病毒载体介导的基因转移目前用于治疗遗传性和获得性血液病的基因治疗方案，以及用于晚期癌症的基因标记和基因治疗方案。*，“尽管人们正在努力优化人类造血干细胞的纯化和转导过程，但外周血淋巴细胞（pbl）仍然被认为是人类基因治疗中最安全的细胞传递载体。许多不同的逆转录病毒载体已经被设计并用于将基因转移到人类造血细胞中，所有这些载体都是基于Moloney小鼠白血病病毒（MoMLV）主干。不同启动子驱动目标基因表达的使用，以及这些序列相对于病毒转录单元的位置，是生成替代载体设计时考虑的一些参数^.^。^其中一些载体用于在不同条件下转导人类淋巴样细胞，例如肿瘤浸润淋巴细胞（TILS）， ‘, ’以及TILS和pbl的CD4+和CD8 '亚群。~表情R
cells (PBMCs), as well as human hematopoietic cell lines of myeloid and lymphoid origin, were transduced with the four vectors and analyzed for efficiency of gene transfer, integration and stability of vector proviruses, and LNGFR expression at both RNA and protein level. Fluorescence-activated cell sorter analysis of coexpression of LNGFR and lineagespecific cell surface markers was performed in transduced ETROVIRAL VECTORS are an efficient and relatively safe tool for the transfer of exogenous DNA into somatic cells.’ Retroviral vector-mediated gene transfer is currently used in gene therapy protocols for the treatment of inherited and acquired blood diseases and in gene marking and gene therapy protocols for advanced cancer.*,’ Although significant effort is being devoted to optimizing procedures for purification and transduction of human hematopoietic stem cells, peripheral blood lymphocytes (PBLs) are still considered the safest cellular delivery vehicle for human gene therapy. A number of different retroviral vectors have been engineered and used for gene transfer into human hematopoietic cells, all based on the Moloney murine leukemia virus (MoMLV) backbone. Usage of different promoters driving expression of the gene of interest, and the position of these sequences with respect to the viral transcription unit, were some of the parameters taken into account in generating alternative vector design^.^.^ Some of these vectors were used to transduce human lymphoid cells under different conditions, eg, tumor-infiltrating lymphocytes (TILS),’,’ and CD4+ and CD8’ subsets of both TILS and PBLs.~ Expression R
影响因子：
2.7
作者：
Miller Ad;Rosman Gj
通讯作者：
Miller Ad;Rosman Gj
通过逆转录病毒介导的基因转移纠正 CD18 缺陷淋巴细胞。
DOI：
10.1126/science.1972597
发表时间：
1990
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Wilson,JM;Ping,AJ;Krauss,JC;Mayo-Bond,L;Rogers,CE;Anderson,DC;Todd,RF
通讯作者：
Todd,RF
影响因子：
2.9
作者：
Wiginton,DA;Kaplan,DJ;States,JC;Akeson,AL;Perme,CM;Bilyk,IJ;Vaughn,AJ;Lattier,DL;Hutton,JJ
通讯作者：
Hutton,JJ