Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individualized brain repair of severe ischemic stroke.
Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individualized brain repair of severe ischemic stroke.
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具有可编程形状记忆特性和神经分化促进活性的可注射丝胶支架用于严重缺血性中风的个体化脑修复
DOI：
10.1016/j.bioactmat.2020.12.017
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发表时间：
2021-07
影响因子：
18.9
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Wang J;Li X;Song Y;Su Q;Xiaohalati X;Yang W;Xu L;Cai B;Wang G;Wang Z;Wang L
严重的缺血性卒中损害神经元组织，形成缺乏支撑结构的不规则形状的卒中空洞。植入生物材料以提供结构和功能支持被认为有利于再生神经元网络的植入。具有原位凝胶能力的可注射水凝胶通常用于中风修复，但存在凝胶不完全和对末端宏观结构控制不精确的问题。可注射的形状记忆支架可能会克服这些限制，但还没有被探索用于中风修复。在这里，我们报道了一种可注射的，发光的，碳纳米管掺杂的丝胶支架(CNTS-SS)，具有可编程的形状记忆特性。通过调整碳纳米管的浓度，碳纳米管-SS的回收动力学可以在秒的尺度上进行数学计算，其形状可以预先设计成与任何不规则形状的空腔精确匹配。使用临床前卒中模型，我们显示定制形状的CNTs-SS被成功地注射到空洞中，并恢复了其预先设计的形状，以很好地适应空洞。值得注意的是，CNTs-SS的近红外光致发光使体内植入后的非侵入性、实时跟踪成为可能。此外，作为细胞载体，CNTs-SS不仅能将骨髓间充质干细胞(BMSCs)送入脑组织，还能在功能上促进其向神经元分化。我们首次证明了可注射形状记忆支架用于卒中修复的可行性，为个性化卒中修复铺平了道路。一种定制的卒中空洞修复策略，使用具有可编程形状记忆特性的预成型丝胶支架。可注射性有助于其体内释放，并将植入损伤降至最低。嵌入的碳纳米管赋予这种生物材料促进神经分化的活性。
Severe ischemic stroke damages neuronal tissue, forming irregular-shaped stroke cavities devoid of supporting structure. Implanting biomaterials to provide structural and functional support is thought to favor ingrowth of regenerated neuronal networks. Injectable hydrogels capable of in situ gelation are often utilized for stroke repair, but challenged by incomplete gelation and imprecise control over end-macrostructure. Injectable shape-memory scaffolds might overcome these limitations, but are not explored for stroke repair. Here, we report an injectable, photoluminescent, carbon-nanotubes-doped sericin scaffold (CNTs-SS) with programmable shape-memory property. By adjusting CNTs' concentrations, CNTs-SS′ recovery dynamics can be mathematically calculated at the scale of seconds, and its shapes can be pre-designed to precisely match any irregular-shaped cavities. Using a preclinical stroke model, we show that CNTs-SS with the customized shape is successfully injected into the cavity and recovers its pre-designed shape to well fit the cavity. Notably, CNTs-SS’ near-infrared photoluminescence enables non-invasive, real-time tracking after in vivo implantation. Moreover, as a cell carrier, CNTs-SS not only deliver bone marrow mesenchymal stem cells (BMSCs) into brain tissues, but also functionally promote their neuronal differentiation. Together, we for the first time demonstrate the feasibility of applying injectable shape-memory scaffolds for stroke repair, paving the way for personalized stroke repair. A customized stroke cavity repairing strategy using preformed sericin-based scaffold with programmable shape-memory properties. Injectability facilitates its' in vivo delivery and minimizes implantation injury. Embedded CNTs endowing this biomaterial neuro-differentiation-promoting activity.
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双重功能可注射的血管生成生物材料，用于中风后修复脑组织。
影响因子：
41.2
作者：
Nih LR;Gojgini S;Carmichael ST;Segura T
通讯作者：
Segura T
DOI：
10.1002/advs.201801046
发表时间：
2018-11
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
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作者：
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通讯作者：
Segura T
影响因子：
2.4
作者：
Jin, Xin;Zhu, Feng;Yang, King H.
通讯作者：
Yang, King H.
DOI：
10.1073/pnas.0508945102
发表时间：
2005-12-13
影响因子：
11.1
作者：
Munoz, JR;Stoutenger, BR;Prockop, DJ
通讯作者：
Prockop, DJ
影响因子：
14.5
作者：
Sakata, Hiroyuki;Narasimhan, Purnima;Chan, Pak H.
通讯作者：
Chan, Pak H.