Isolation of bovine angiogenin using a placental ribonuclease inhibitor binding assay.
Isolation of bovine angiogenin using a placental ribonuclease inhibitor binding assay.
复制标题
使用胎盘核糖核酸酶抑制剂结合测定分离牛血管生成素。
DOI：
10.1021/bi00417a013
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
B. Vallée
中科院分区：
文献类型：
--
作者：
M. D. Bond;B. Vallée
血管生成素是从两个人类来源--HT-29肿瘤条件培养液和正常血浆中分离出来的，它能诱导新血管的形成。通过使用新开发的结合分析，现在已经从牛血浆中纯化了类似的蛋白质，水平为30-80微克/L。该蛋白质具有血管生成素分子所期望的结构、酶和生物学特性。它的氨基酸组成与人类蛋白质相似，在31个氨基酸末端序列中有22个是相同的，包括11个连续残基。与人类血管生成素一样，牛蛋白与胎盘核糖核酸酶抑制物结合，对传统的RNaseA底物不起作用，并对某些rRNA显示选择性的核糖核溶解活性。此外，牛蛋白在鸡胚绒毛膜尿囊膜实验中诱导体内血管生成的水平低至每个鸡蛋44fmoL。因此，血管生成素在牛血清中的存在水平与在人类中观察到的水平相似，其酶活性和生物学活性与人类蛋白质的活性相同。
Angiogenin, which induces the formation of new blood vessels, was isolated previously from two human sources--HT-29 tumor conditioned media and normal plasma. By use of a newly developed binding assay, a similar protein has now been purified from bovine plasma at levels of 30-80 micrograms/L. This protein has the structural, enzymatic, and biological characteristics expected for an angiogenin molecule. Its amino acid composition is similar to that of the human protein, and 22 of 31 residues in the amino-terminal sequences are identical, including a block of 11 consecutive residues. Like human angiogenin, the bovine protein binds placental ribonuclease inhibitor, is inactive toward conventional RNase A substrates, and displays selective ribonucleolytic activity toward some rRNAs. In addition, the bovine protein induces angiogenesis in vivo in the chick embryo chorioallantoic membrane assay at levels as low as 44 fmol per egg. Thus, angiogenin is present in bovine sera at levels similar to those observed in man, and its enzymatic and biological activities are identical with those of the human protein.
DOI：
--
发表时间：
1979-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Blackburn
通讯作者：
P. Blackburn
影响因子：
2.9
作者：
BLANK, A;DEKKER, CA
通讯作者：
DEKKER, CA
影响因子：
2.9
作者：
TARENTINO, AL;GOMEZ, CM;PLUMMER, TH
通讯作者：
PLUMMER, TH