Spermiogenesis in Cancer crabs.
Spermiogenesis in Cancer crabs.
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DOI：
10.1083/jcb.43.3.575
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发表时间：
1969-12
期刊：
影响因子：
--
通讯作者：
Langreth SG
中科院分区：
文献类型：
--
作者：
Langreth SG
用光镜和电镜对蟹的精子发生进行了研究。精子是鞭状的，成熟时主要由一个球形顶体组成，顶体被核和它的短辐射臂包围。顶体由周期性酸-希夫阳性（pas阳性）囊泡合并形成。在精子发生过程中，顶体囊泡的一个边缘内陷形成pas阴性的中央核。顶体与核心结合的内部区域含有不与核酸络合的基本蛋白质。描述了主要由内质网膜形成的复杂的融合膜的晶格状复合体。这些膜随后被带入细胞核并随后退化。在后期精子细胞中，当大部分细胞质脱落时，核膜和细胞膜明显融合，成为大部分精子细胞的边界。在成熟的精子中，核和臂的染色质是feulgen阳性的，不含可检测到的蛋白质。染色质丝呈团块状、分枝状和吻合状；在形态上，它们类似于细菌细胞核的DNA。巨蟹成熟精子中线粒体缺失或退化，而中心粒存在于顶体基部的核质中。
Spermiogenesis in Cancer crabs was studied by light and electron microscopy. The sperm are aflagellate, and when mature consist primarily of a spherical acrosome surrounded by the nucleus with its short radiating arms. The acrosome forms by a coalescence of periodic acid-Schiff-positive (PAS-positive) vesicles. During spermiogenesis one edge of the acrosomal vesicle invaginates to form a PAS-negative central core. The inner region of the acrosome bounding the core contains basic proteins which are not complexed to nucleic acid. The formation of an elaborate lattice-like complex of fused membranes, principally from membranes of the endoplasmic reticulum, is described. These membranes are later taken into the nucleus and subsequently degenerate. In late spermatids, when most of the cytoplasm is sloughed, the nuclear envelope and the cell membrane apparently fuse to become the limiting boundary over most of the sperm cell. In the mature sperm the chromatin of the nucleus and arms, which is Feulgen-positive, contains no detectable protein. The chromatin filaments appear clumped, branched, and anastomosed; morphologically, they resemble the DNA of bacterial nuclei. Mitochondria are absent or degenerate in mature sperm of Cancer crabs, but the centrioles persist in the nucleoplasm at the base of the acrosome.
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影响因子：
--
作者：
Bowen, RH
通讯作者：
Bowen, RH
DOI：
10.1083/jcb.7.3.515
发表时间：
1960-01-01
期刊：
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY
影响因子：
--
作者：
BLOCH, DP;HEW, HYC
通讯作者：
HEW, HYC
DOI：
10.1083/jcb.2.3.223
发表时间：
1956-01-01
期刊：
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY
影响因子：
--
作者：
BURGOS, MH;FAWCETT, DW
通讯作者：
FAWCETT, DW
影响因子：
--
作者：
Fasten, N
通讯作者：
Fasten, N
DOI：
10.1086/physzool.25.4.30152126
发表时间：
1952-01-01
期刊：
PHYSIOLOGICAL ZOOLOGY
影响因子：
--
作者：
FLAX, MH;HIMES, MH
通讯作者：
HIMES, MH