Evaluation of the antioxidant activity of cell extracts from microalgae.
Evaluation of the antioxidant activity of cell extracts from microalgae.
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DOI：
10.3390/md11041256
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发表时间：
2013-04-17
期刊：
影响因子：
5.4
通讯作者：
Malcata FX
中科院分区：
文献类型：
--
作者：
Guedes AC;Gião MS;Seabra R;Ferreira AC;Tamagnini P;Moradas-Ferreira P;Malcata FX
从非昂贵来源获得的新型抗氧化剂的市场不断增长，证明了微藻潜在的抗氧化剂特性的筛选是合理的。本文对18种蓝藻（原核微藻）和23种（真核微藻）的抗氧化特性进行了研究。总的抗氧化能力，占水溶性和脂溶性抗氧化剂，（自由基阳离子）ABTS法进行了评价。对于细胞提取物的补充表征，进行脱氧核糖测定，以及噬菌体P22/沙门氏菌介导的方法。微藻栅藻M2-1细胞内提取物的总抗氧化能力最高（149 ± 47 AAU）（p > 0.05）。它的清除剂活性相关以及其对铜（II）-抗坏血酸诱导的DNA氧化损伤的保护作用;和对腐败的噬菌体感染能力诱导过氧化氢。最后，艾姆斯试验表明，所述浸提液无致突变作用。
A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation) ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05) total antioxidant capacity (149 ± 47 AAU) of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II)-ascorbic acid; and against decay in bacteriophage infection capacity induced by H2O2. Finally, performance of an Ames test revealed no mutagenic effects of the said extract.
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影响因子：
4.3
作者：
Hong, Yu;Hu, Hong-Ying;Li, Feng-Min
通讯作者：
Li, Feng-Min
影响因子：
8.8
作者：
Hu, Chao-Chin;Lin, Jau-Tien;Yang, Deng-Jye
通讯作者：
Yang, Deng-Jye
DOI：
10.1073/pnas.70.8.2281
发表时间：
1973-01-01
影响因子：
11.1
作者：
AMES, BN;DURSTON, WE;LEE, FD
通讯作者：
LEE, FD
影响因子：
7.4
作者：
Muñiz, P;Sáez, P;Sáez, GT
通讯作者：
Sáez, GT
影响因子：
3.9
作者：
Guimaraes, Carla M.;Giao, Maria S.;Malcata, F. Xavier
通讯作者：
Malcata, F. Xavier