Tolerance to isoflurane does not occur in developing Xenopus laevis tadpoles.
Tolerance to isoflurane does not occur in developing Xenopus laevis tadpoles.
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DOI：
10.1213/ane.0b013e31818ca33e
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发表时间：
2009-01
影响因子：
5.7
通讯作者：
Sonner JM
中科院分区：
文献类型：
--
作者：
Milutinovic PS;Zhao J;Sonner JM
观察到对多种中枢神经系统抑制剂的耐受性，包括乙醇，乙醇是一种麻醉剂，但尚未令人信服地证明有效的卤代挥发性麻醉剂。未能证明对这些药物的耐受性可能是麻醉剂暴露不足的结果。在这项研究中，我们将非洲爪蟾蝌蚪暴露于手术麻醉浓度的异氟烷中一周。非洲爪蟾蝌蚪通过体外受精产生，并从受精时开始暴露于异氟醚（0.59%，0.98%，1.52%）或氧气一周。麻醉剂EC50的变化较小，且方向不一致。对照动物的麻醉剂EC50为0.594% ± 0.003%异氟烷。暴露于1.52%异氟烷的蝌蚪的EC 50低于对照组（16%），而在0.59和0.98%异氟烷下饲养的蝌蚪的EC 50高于对照组（分别为4.7%和7.4%）。我们提供了第一个描述的脊椎动物为期一周的暴露于手术麻醉浓度的异氟烷，并在发展中的脊椎动物的这种风险的第一份报告。异氟烷耐受性不会发生在非洲爪蟾蝌蚪的发展。与其他生物体中的研究一起，对乙醇而非异氟烷的耐受性的发展表明这些药物共有的机制可能不能解释耐受性的发展。
Tolerance is observed for a variety of central nervous system depressants including ethanol, which is an anesthetic, but has not been convincingly demonstrated for a potent halogenated volatile anesthetic. Failure to demonstrate tolerance to these agents may be the result of inadequate exposure to anesthetic. In this study, we exposed Xenopus laevis tadpoles to surgical anesthetic concentrations of isoflurane for one week. Xenopus laevis tadpoles were produced by in vitro fertilization, and exposed to isoflurane (0.59%, 0.98%, 1.52%) or oxygen for one week starting from the time of fertilization. Changes in anesthetic EC50 were small and not in a consistent direction. Control animals had an anesthetic EC50 of 0.594% ± 0.003% isoflurane. Tadpoles exposed to 1.52% isoflurane had a lower EC50 than controls (by 16%), while tadpoles raised under 0.59 and 0.98% isoflurane had higher EC50s than control (by 4.7% and 7.4%, respectively). We provide the first description of week-long exposures of vertebrates to surgical anesthetic concentrations of isoflurane, and the first report of such exposures in developing vertebrates. Tolerance to isoflurane does not occur in developing Xenopus laevis tadpoles. Taken together with studies in other organisms, the development of tolerance to ethanol but not isoflurane suggests that mechanisms these drugs share probably do not account for the development of tolerance.
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影响因子：
8.8
作者：
LUTZ, LJ;MILDE, JH;MILDE, LN
通讯作者：
MILDE, LN
DOI：
10.1016/0005-2736(89)90009-6
发表时间：
1979-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
NANDINIKISHORE, SG;MATTOX, SM;THOMPSON, GA
通讯作者：
THOMPSON, GA
影响因子：
64.8
作者：
Rudolph, U;Crestani, F;Möhler, H
通讯作者：
Möhler, H
影响因子：
13.8
作者：
Chandler, LJ;Harris, RA;Crews, FT
通讯作者：
Crews, FT
影响因子：
7.3
作者：
Mohr, JT;Gribble, GW;Cantor, RS
通讯作者：
Cantor, RS